Thermal inactivation kinetics of microencapsulated microbial transglutaminase by ultrasonic spray-freeze drying      
Yazarlar (2)
Prof. Dr. Hilal İŞLEROĞLU Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Arş. Gör. İzzet TÜRKER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Lwt
Dergi ISSN 0023-6438 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 03-2019
Cilt No 101
Sayı 1
Sayfalar 653 / 662
DOI Numarası 10.1016/j.lwt.2018.11.091
Makale Linki https://linkinghub.elsevier.com/retrieve/pii/S0023643818310521
Özet
Partially purified microbial transglutaminase (mTG) was microencapsulated by ultrasonic spray-freeze drying (USFD) as a novel method, using a 48 kHz ultrasonic nozzle. The microencapsulated samples, which used a mixture of inulin and gum arabic as coating materials, were compared to microencapsulated samples by conventional freeze-drying (CFD) and conventional spray drying (CSD) in terms of thermal stability. Thermal inactivation kinetics of the microencapsulated and as well as crude enzymes obtained by USFD process (USFD-crude) were evaluated according to first-order reaction kinetics in the range of 40–60 °C at different pH values (5.0–7.0), and the activation energy (Ea, kJ/mol) and free energy for thermal inactivation (ΔG, kJ/mol) were calculated. Furthermore, the specific surface area (SSA, m2/g) of the samples was determined, and a relationship between SSA and thermal stability was established. The results showed that the thermal stability of microencapsulated mTG by USFD was higher than all samples, having smaller rate constants and Ea values with higher half-life times and ΔG values. In addition, the thermal stability of the samples decreased when the SSA increased, as in USFD-crude and CSD samples.
Anahtar Kelimeler
Microbial transglutaminase | Microencapsulation | Specific surface area | Thermal inactivation | Ultrasonic spray-freeze drying