Modulation of oxidative stress apoptosis and calcium entry in leukocytes of patients with multiple sclerosis by Hypericum perforatum
Yazarlar (5)
Süleyman Demirel Üniversitesi, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
Doç. Dr. Mehmet AYKUR
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Nutritional Neuroscience |
| Dergi ISSN | 1028-415X Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2014 |
| Cilt No | 17 |
| Sayı | 5 |
| Sayfalar | 214 / 221 |
| DOI Numarası | 10.1179/1476830513Y.0000000083 |
| Makale Linki | http://www.maneyonline.com/doi/abs/10.1179/1476830513Y.0000000083 |
| Özet |
| Objectives: Hypericum perfortarum (HP, St John's wort) is a modulator of Ca2+ entry in neutrophils and it may modulate intracellular free Ca2+ ([Ca2+]i) entry in leukocytes of patients with multiple sclerosis (MS). We investigated effects of HP on oxidative stress, apoptosis, and [Ca2+]i concentrations in serum and leukocytes of patients with MS. Methods: Neutrophils of nine newly diagnosed MS patients and nine healthy subjects within four subgroups were used in the study. The first group was a control; the second group was patients with MS. The neutrophils from patient group were incubated non-specific TRPM2 channel blocker (2-APB), voltage-gated calcium channel blockers, verapamil and diltiazem (V + D) with HP before N-formyl-L-methionyl-L-leucyl-L-phenylalanine stimulation, respectively. Results: Neutrophil and serum lipid peroxidation, neutrophil apoptosis and [Ca2+]i levels in patients with MS were higher than in control although their levels were decreased by HP, 2-APB, and V + D incubations. The modulator role of V + D in MS and MS + HP groups was higher than in the 2-APB group. Neutrophilic glutathione peroxidase (GSH-Px) and serum vitamin A and E concentrations were lower in the MS group than in control. However, the neutrophil GSH-Px activity was increased by HP incubation. The neutrophil reduced glutathione, serum vitamin C and β-carotene concentrations did not change in control and patients. Discussion: We observed that HP-induced protective effects on oxidative stress and [Ca2+]i concentrations by modulating transient receptor potential and voltage gated calcium channel in the patients with MS. Thus, it may provide useful treatment of neutrophil activity in the patients. © W. S. Maney & Son Ltd 2014. |
| Anahtar Kelimeler |
| Inflammation | Multiple sclerosis | Neutrophils | Oxidative stress | TRP channels |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 29 |
| SCOPUS | 33 |
| Google Scholar | 49 |