Construction of a multiepitope vaccine candidate against Fasciola hepatica: an in silico design using various immunogenic excretory/secretory antigens
Yazarlar (5)
Istanbul Medeniyet University, Türkiye
Doç. Dr. Mehmet AYKUR
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Expert Review of Vaccines |
| Dergi ISSN | 1476-0584 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2022 |
| Cilt No | 21 |
| Sayı | 7 |
| Sayfalar | 993 / 1006 |
| DOI Numarası | 10.1080/14760584.2022.1996233 |
| Özet |
| Background: Fasciola hepatica is an important pathogen that causes liver fluke disease in definitive hosts such as livestock animals and humans. Various excretory/secretory products have been used in serological diagnosis and vaccination studies targeting fasciolosis. There are no commercial vaccines against fasciolosis yet. Bioinformatic analysis based on computational methods have lower cost and provide faster output compared to conventional vaccine antigen discovery techniques. The aim of this study was to predict B- and T-cell specific epitopes of four excretory/secretory antigens (Kunitz-type serine protease inhibitor, cathepsin L1, helminth defense molecule, and glutathione S-transferase) of Fasciola hepatica and to construct a multiepitope vaccine candidate against fasciolosis. Methods and Results: Initially, nonallergic and the highest antigenic B- and T- cell epitopes were selected and then, physico-chemical parameters, secondary and tertiary structures of designed multiepitope vaccine candidate were predicted. Tertiary structure was refined and validated using online bioinformatic tools. Linear and discontinuous B-cell epitopes and disulfide bonds were determined. Finally, molecular docking analysis for MHC-I and MHC-II receptors was performed. Conclusion: This multi-epitope vaccine candidate antigen, with high immunological properties, can be considered as a promising vaccine candidate for animal experiments and wet lab studies. |
| Anahtar Kelimeler |
| excretory/secretory (E/S) antigens | Fasciola hepatica | in silico analysis | multiepitope vaccine | vaccine development |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 14 |
| SCOPUS | 14 |
| Google Scholar | 18 |