Synthesis and biological evaluation of radiolabeled photosensitizer linked bovine serum albumin nanoparticles as a tumor imaging agent
Yazarlar (5)
Doç. Dr. Aykut ÖZGÜR
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Tarsus Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | International Journal of Pharmaceutics |
| Dergi ISSN | 0378-5173 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2012 |
| Cilt No | 422 |
| Sayı | 1 |
| Sayfalar | 472 / 478 |
| DOI Numarası | 10.1016/j.ijpharm.2011.11.013 |
| Makale Linki | https://www.sciencedirect.com/science/article/abs/pii/S0378517311010519 |
| Özet |
| In this study, we reported on the synthesis and biological evaluation of radiolabeled fluorescent dye conjugated bovine serum albumin nanoparticles within the size range 190-210 nm. The bovine serum albumin nanoparticles (BSANPs) were prepared using a desolvation method, and chemical cross-linking was performed using gluteraldehyde. Furthermore, pheophorbide-a (PH-A) was loaded on the BSANPs. The results obtained from dynamic light scattering and electron microscopy have proved that nanoparticles are highly monodisperse and near-spherical shaped. The photo-physical properties of the PH-A-BSANPs were obtained using the spectrophotometric techniques. According to the results, PH-A and BSANPs show high non-covalent interaction. PH-A loaded nanoparticles were labeled with 99mTc and the radio-labeling efficiency was determined as 90 ± 1.2%. Biodistribution studies of 99mTc labeled PH-A-BSANPs and PH-A were carried out using female Albino Wistar rats, and 99mTc-PH-A-BSANPs showed a significantly higher uptake in the breast and uterus than 99mTc-PH-A. Cell culture study was carried out in MCF-7 cell line (human breast adenocarcinoma cell line). According to the cell culture studies, 99mTc-PH-A-BSANPs showed a higher uptake than 99mTc-PH-A. Moreover, PH-A-BSANPs demonstrated good photo-physical properties and BSANPs increased the uptake of PH-A on to the MCF-7 cell line. These results confirm that 99mTc labeled PH-A-BSANPs could be utilized for radioimaging. © 2011 Elsevier B.V. |
| Anahtar Kelimeler |
| 99m Tc | BSA nanoparticles | Drug delivery systems | MCF-7 | Pheophorbide-a |