Fenugreek seed proteins: Ultrasonic‐assisted extraction, characterization, and cupcake application
Yazarlar (3)
Arş. Gör. İzzet TÜRKER
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Hilal İŞLEROĞLU
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Food Science and Nutrition |
| Dergi ISSN | 2048-7177 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | Türkçe |
| Basım Tarihi | 06-2024 |
| Cilt No | 12 |
| Sayı | 9 |
| Sayfalar | 6353 / 6366 |
| DOI Numarası | 10.1002/fsn3.4247 |
| Makale Linki | http://dx.doi.org/10.1002/fsn3.4247 |
| Özet |
| In this study, fenugreek seed proteins were extracted using ultrasonic-assisted extraction with varying solid:solvent ratios (20–60 g/L) and sonication amplitudes (30%–80%) to determine optimal conditions for the highest extraction yield. The functional, structural, and nutritional characteristics of the protein isolates of fenugreek seeds were investigated. The highest yield (98.74 ± 0.49%) was achieved at a solid:solvent ratio of 43.83 g/L and an amplitude of 67.51%. The coagulated protein values of fenugreek seed protein isolates ranged from ~15.8% to 31.2%, water-holding capacities ranged from ~2.2 to 3.2 g/g, oil-holding capacities ranged from ~2.6 to 4.1 g/g, foaming capacities ranged from ~16.3% to 21.3%, foam stabilities ranged from ~59.7% to 78.1%, emulsion stabilities ranged from ~30.2 to 34.5 min, emulsion activities ranged from ~73.8 to 76.8 m2/g, and emulsion capacities ranged from ~26.9% to 30.5% under different extraction conditions. SDS-PAGE analysis revealed three distinct bands (46, 59, and 80 kDa) for the protein isolates. FT-IR spectroscopy showed a high presence of β-sheet structures. The amino acid composition analysis of fenugreek seed protein isolates was determined, revealing richness in essential amino acids (317.97 g amino acid/kg protein isolate). In addition, cupcakes enriched with protein isolates (5%, 10%, and 20% as flour substitutes) were produced, and quality properties such as color change, browning index, moisture content, water activity, baking yield, bulk density, hardness, volume, symmetry, and uniformity indexes were determined. The application of protein isolates in cupcake production demonstrated the potential of fenugreek seeds as valuable ingredients for enhancing the nutritional profile of bakery products. |
| Anahtar Kelimeler |
| coagulated protein | cupcake | fenugreek seeds | ultrasound-assisted extraction | β-Sheet |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 4 |
| Google Scholar | 4 |