Staining of Onion and Buccal Epithelial Cells with Onion Skin Exract   
Yazarlar (3)
Nilgün Kuşçulu
Kayseri Üniversitesi, Türkiye
Nurhan Cücer
Erciyes Üniversitesi, Türkiye
Prof. Dr. Adem ÖNAL Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Makale Türü Özgün Makale
Makale Alt Türü Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale
Dergi Adı Research Journal of Biology
Dergi ISSN 2322-0066
Dergi Tarandığı Indeksler Academic Journals Database, academic resource index, citefactor, index copernicus, open-J gate, GIF, Cosmos
Makale Dili İngilizce
Basım Tarihi 04-2017
Cilt No 5
Sayı 2
Sayfalar 1 / 6
Makale Linki https://www.rroij.com/open-access/staining-of-onion-and-buccal-epithelial-cells-with-onion-skin-extract-.php?aid=85732
Özet
The aim of our study is to reveal the stainability of different parts of buccal epithelial cells as an animal cell model, and onion membrane as a plant cell model with extract prepared from onion skin by means of light microscope images. After a 20 gram amount of the skins of clean and fresh headed onions with orange-yellow outer skins is ground, it is taken with precision weighing. This is added to 100 ml of purified water, boiled for 30 minutes, and filtered. The filtrate is stored in the refrigerator at +4°C for use as a dye solution. Solvents with separate binary combinations from the dye solutions alone and various metal salts (Alum, FeSO4, CuSO4) were prepared. Membrane pieces in the onion and buccal epithelial preparations were put into each solution. The staining process is finalized by keeping the prepared materials at 50°C and 80°C in an incubator for 1 hour, and then at room temperature for 1 hour. The onion …
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