Enhancing sensitivity of chemoresistant ovarian cancer cells to TRAIL and FAS mediated apoptosis by radiation
Yazarlar (1)
Prof. Dr. Ercan ÇAÇAN
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale |
| Dergi Adı | Turk Hijyen Ve Deneysel Biyoloji Dergisi |
| Dergi ISSN | 0377-9777 Scopus Dergi |
| Dergi Tarandığı Indeksler | TR DİZİN |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2017 |
| Cilt No | 74 |
| Sayı | 3 |
| Sayfalar | 185 / 192 |
| DOI Numarası | 10.5505/TurkHijyen.2017.12499 |
| Özet |
| Objective: Death receptors initiate apoptotic signals following interaction with their cognate ligands. However, expressions of death receptors are often downregulated during ovarian cancer progression and it has been recently asserted that suppression of death receptors is associated with resistance to chemotherapeutic drugs in ovarian cancer cells. Radiotherapy is a common treatment modality for several cancer types and it has been reported that low-dose ionizing radiation modulates tumor microenvironment. The purpose of the present study is to determine if sub-lethal ionizing radiation will modulate the expression of common death receptors in chemoresistant ovarian cancer cells and to investigate if reversed expression of death receptors will enhance TRAIL or FAS ligand (FASL) mediated apoptosis. Methods: Flow cytometry analyses were performed to determine the effects of chemotherapeutic drug, cisplatin, on chemosensitive and chemoresistant ovarian cancer cells viability, cellular expressions of death receptors and TRAIL or FAS mediated apoptosis, following sub-lethal irradiation in drug resistant ovarian cancer cells. Results: The majority of chemoresistant A2780-AD cells remain viable following a high dose of cisplatin treatment, while the drug sensitive A2780 cells started to die following low dose drug exposure. The results have demonstrated that 2 Gy or 5 Gy ionizing radiation enhances expression of death receptors, FAS and DR4, in multi drug resistant A2780-AD ovarian cancer cells. The data further have confirmed that sub-lethal ionizing radiation increases FAS/TRAIL-mediated apoptosis of the chemoresistant ovarian tumor cells. Conclusion: This study has suggested that sublethal radiation treatment may simultaneously increase immunogenicity of tumor cells and the induction of antitumor immunity to chemoresistant ovarian cancer cells. |
| Anahtar Kelimeler |
| Chemoresistance | FAS | Ovarian cancer | Radiation | TRAIL |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| SCOPUS | 2 |
| Google Scholar | 3 |