Synthesis and characterization of silver nanoparticles using Origanum onites leaves: Cytotoxic, apoptotic, and necrotic effects on Capan-1, L929, and Caco-2 cell lines
Yazarlar (1)
Doç. Dr. Esma Nur GEÇER
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Green Processing and Synthesis |
| Dergi ISSN | 2191-9542 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2023 |
| Cilt No | 12 |
| Sayı | 1 |
| DOI Numarası | 10.1515/gps-2022-8126 |
| Makale Linki | http://dx.doi.org/10.1515/gps-2022-8126 |
| Özet |
| In this study, Origanum onites was used to synthesize the silver nanoparticles (AgNPs@Org). The structure of nanoparticles was identified by spectroscopic techniques. The maximum absorption was determined as 433 nm by UV-Vis spectroscopy. In Fourier-Transform infrared spectroscopy spectrum, the characteristic signal was observed at 3,262 cm-1 belonging to the OH group. The crystal structure of nanoparticles was revealed by X-ray diffraction analysis. The diffraction peaks (2θ) can be indexed to 111, 200, 220, 311, and 222 components representing the face-centered cubic unit structure. The spherical particle size was calculated as 18.1 nm by transmission electron microscopy. Cytotoxic effects of extract and AgNPs@Org were executed by MTT assay using Capan-1, L929, and Caco-2 cell lines. AgNPs@Org exhibited the excellent cytotoxic effect on Capan-1 cell lines with the viability of 37.6% (0.5 μg·mL-1). However, the effect of O. onites extract on the viability of Capan-1 cell lines was found to be 24.6% and 55.4% at 1.0 and 0.5 μg·mL-1, respectively. AgNPs@Org effect on Caco-2 cell lines was found as 31.7% (1.0 μg·mL-1). In the L929 cell lines, the noticeable lethal influence was not detected for extract and nanoparticles. In other words, the extract and AgNPs@Org did not act a cytotoxic effect on L929 cell lines. |
| Anahtar Kelimeler |
| silver nanoparticles | spectroscopy | Origanum onites | anticancer activity | apoptosis |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 8 |
| SCOPUS | 10 |
| Google Scholar | 11 |