Recovery and Reusability of ApoUnaG Fluorescence Protein from the Unconjugated Bilirubin Complex Structure
Yazarlar (4)
Dr. Öğr. Üyesi Numan ECZACIOĞLU Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Dr. Öğr. Üyesi Bahar Yilmaz Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Prof. Dr. Yakup Ulusu Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Prof. Dr. Mevlut Bayrakci Karamanoğlu Mehmetbey Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Fluorescence (Q3) | ||
| Dergi ISSN | 1053-0509 Dergi Bilgileri (2020) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2020 |
| Cilt / Sayı / Sayfa | 30 / 3 / 497–503 | DOI | 10.1007/s10895-020-02519-w |
| Makale Linki | http://link.springer.com/10.1007/s10895-020-02519-w | ||
| UAK Araştırma Alanları |
Fen Bilimleri ve Matematik
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| Özet |
| This study is the first report on the separation and reusability of ApoUnaG protein, indicating excellent fluorescence response with high affinity and specificity toward unconjugated bilirubin (UC-BR) molecules, from the UnaG-UC-BR complex structure. The fluorescence properties of the UnaG-UC-BR complex (holo-UnaG) are studied by addition of different metal ions to perform possible interactions with holo-UnaG through absorbance and emission spectra. After addition of metal ions, some changes with respect to the type of metal ions are observed in fluorescence intensity of the holo-UnaG. When compared to metal ions, an excellent quenching response is sighted in the presence of Cu2+ ions by binding with UC-BR in the UnaG-UC-BR complex structure. Obtained non-fluorescence holo-UnaG-Cu2+ complex mixture is passed through Ni–NTA agarose to remove the ingredients such as Cu2+, UC-BR and Cu2 … |
| Anahtar Kelimeler |
| Bilirubin | Copper | Fluorescence quenching | UnaG |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Yöksis | 2 |
| Web of Science | 1 |
| Scopus | 1 |
| Google Scholar | 3 |