Biochemical and Molecular Characterization of Wheat Genotypes Under Drought Stress: Implications for Antioxidant Defense Mechanisms and Genomic Analysis
Yazarlar (4)
Dr. Öğr. Üyesi Numan ECZACIOĞLU Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Dr. Öğr. Üyesi Begüm Terzi Aksoy Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Prof. Dr. Özlem Ateş Sönmezoğlu Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Prof. Dr. Yakup Ulusu Karamanoğlu Mehmetbey Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale)
Dergi Adı Acta Agriculturae Slovenica
Dergi ISSN 1581-9175 Dergi Bilgileri (2024)
Dergi Tarandığı Indeksler Scopus
Makale Dili Türkçe Basım Tarihi 10-2024
Cilt / Sayı / Sayfa 120 / 3 / 1–0 DOI 10.14720/aas.2024.120.3.18268
Makale Linki https://doi.org/10.14720/aas.2024.120.3.18268
UAK Araştırma Alanları
Özet
In abiotic stress factors, drought is a complex feature controlled by many genes. For this reason, drought stress effects should be investigated using molecular markers and biochemical characterization in wheat genotypes. Within the scope of this study; eight different wheat cultivars and two for control group including Gerek-79, known to be highly tolerant of drought, and sensitive Sultan-95 were grown during 40 days. After breeding, they were subjected to drought stress for 10 days. As a result of water deficiency stress, antioxidant amounts and antioxidant enzyme activities, which convert ROS into harmless components, are the most important resistance mechanisms against oxidative stress. Among the most effective anti-oxidative enzymes such as polyphenol oxidase (PPA), peroxidase (POD), ascorbate peroxidase (ASPX), catalase (CAT) levels were determined. The response of plants to stress can be described as changes in photosynthetic pigment, total protein, hydrogen peroxide, lipid peroxidation (MDA) and proline levels as well as antioxidant enzymes level. We also analyzed wheat genotypes with drought tolerance related SSR markers (Xwmc 89, Xwmc 118, Xwmc 304, Xgwm 337). Thus, the effect of molecular characterization on the analysis of biochemical changes under drought stress was evaluated.
Anahtar Kelimeler
drought stress | antioxidant enzymes