Determination of Antioxidant Activity and Phenolic Compound Content of Black Carrot Callus Culture
Yazarlar (5)
Dr. Öğr. Üyesi İlhami KARATAŞ Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Fatih POLAT Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Rahime Karataş
Öğr. Gör. Tarık DAL Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Mahfuz Elmastaş Sağlık Bilimleri Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
Dergi Adı Ecological Life Sciences
Dergi ISSN 1308-7258
Dergi Tarandığı Indeksler ARI, CIF, CrossRef, DOI, DRJI, ESJI, Google Scholar, PILA, SIS
Makale Dili İngilizce Basım Tarihi 04-2018
Kabul Tarihi – Yayınlanma Tarihi 01-04-2018
Cilt / Sayı / Sayfa 13 / 2 / 87–96 DOI 10.12739/NWSA.2018.13.2.5A0097
Makale Linki http://dx.doi.org/10.12739/nwsa.2018.13.2.5a0097
UAK Araştırma Alanları
Bitki Biyoteknoloji Biyokimya
Özet
Callus culture, a method of plant tissue culture, is an alternative technique for the production of phenolic compounds that many industries need. In this study, antioxidant capacity, total phenolic, flavonoid and anthocyanin content of callus obtained from black carrot (Daucus carota ssp. Sativus var. Atrorubens alef.) were investigated. The antioxidant capacities of the extracts were determined by the free radical scavenging activity (DPPH) and the ferric reducing antioxidant power (FRAP) methods. The callus was derived from the MS medium (Murashige ve Skoog containing 2 mg/L 2.4-D, 0.2mg/L BAP, 30g/L sucrose and 2g/L phytagel using hypocotyl segments of plants grown in sterile conditions. The black carrot calli exhibited high DPPH free radical scavenging activity and FRAP activity in addition to high phenolic compound contents.
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