A New Culturing Method for The Rice White Tip Nematode Aphelenchoides besseyi Christie 1942 on Carrot Discs
Yazarlar (11)
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Russian Journal of Nematology (Q4) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2009 |
| Cilt / Sayı / Sayfa | 17 / 2 / 135–136 | DOI | – |
| Özet |
| The rice white tip nematode, Aphelenchoides besseyi, first described by Christie (1942), belongs to the Class Aphelenchida and family Aphelenchoididae. It was discovered by Kakuta in 1915 (Van Nieuwenhuyzen, 1977; Mc Gawley et al., 1984). According to Franklin and Siddiqi (1972), the species is a synonym of the pest Aphelenchoides oryzae Y okoo, 1948. Aphelenchoides besseyi has been reported from USA, Africa, Bangladesh, El Salvador, Indonesia, India, Italy, Korea, Cuba, Hungary, Pakistan, Taiwan and Russia (Franklin and Siddiqi, 1972). In Turkey it was first reported in 1995 in Ipsala (Edirne) and Gonen (Balikesir)(Ozturk and Enneli, 1997). Typical symptoms of this nematode are found only on the aboveground parts of the rice plant and include 3 to 5 cm length of the tip of the leaves turning white, the panicle leaf often becoming twisted and curled, and a shorter panicle that is often atrophied at the tips (Figure 1 a, b, c). Genetic resistance offers one of the best control methods for A. besseyi; however, laboratory screening for resistance requires mass rearing of the nematode. Multiplication of A. besseyi was achieved using carrot discs in the laboratory, which has been reported by Moody et al.(1973) to work successfully with the root lesion nematodes Pratylenchus vu/nus. Using this method, an average of 10,000 A. besseyi individuals can be obtained from a carrot culture in approximately 4 months. The protocol used to produce these cultures is a modification of the published method by Moody et al.(1973) and requires newly harvested fresh, healthy carrots. These were peeled in a laminar flow cabinet using a sterile peeler which … |
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