Optimization of polyphenol extraction, phenolic profile by LC-ESI-MS/MS, antioxidant, anti-enzymatic, and cytotoxic activities of Physalis acutifolia
Yazarlar (13)
University Mohamed Boudiaf Of M‘Sila, Cezayir
University Mohamed Boudiaf Of M‘Sila, Cezayir
Université De Jijel, Cezayir
Centre De Recherche En Biotechnologie, Cezayir
Doç. Dr. İlyas YILDIZ
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ondokuz Mayıs Üniversitesi, Türkiye
University Mohamed Boudiaf Of M‘Sila, Cezayir
Universitatea Alexandru Ioan Cuza, Romanya
Imam Mohammad Ibn Saud Islamic University, Suudi Arabistan
Imam Mohammad Ibn Saud Islamic University, Suudi Arabistan
Princess Nourah Bint Abdulrahman University, Suudi Arabistan
Mersin Üniversitesi, Türkiye
Imam Mohammad Ibn Saud Islamic University, Suudi Arabistan
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Open Chemistry |
| Dergi ISSN | 2391-5420 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2024 |
| Cilt No | 22 |
| Sayı | 1 |
| Sayfalar | 20240040 / 0 |
| DOI Numarası | 10.1515/chem-2024-0040 |
| Makale Linki | https://doi.org/10.1515/chem-2024-0040 |
| Özet |
| During the current study, the phenolic and flavonoid contents were measured in Physalis acutifolia extracts with Folin-Ciocalteu and AlCl3methods, respectively. Various antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl, ABTS, reducing power, Fe2+-phenanthroline reduction, and silver nanoparticle assays, were also conducted, along with anti-enzymatic assays. The cytotoxicity of the ethanolic extract was assessed on cancer cell lines (i.e., CAPAN-1 and dld-1) and a healthy cell line (i.e., L929). Optimal parameters for polyphenol extraction were determined: extraction time of 72 h, solvent-to-plant ratio of 10 mL/1 g, and plant concentration of 90%. The n-butanol extract showed the highest phenolic and flavonoid contents (i.e., 263.84 mg GAE/g dw and 72.03 QE/g dw, respectively), along with superior antioxidant and anti-enzymatic activities with IC50values of 49.77 and 187.12 μg/mL with acetylcholinesterase and butyrylcholinesterase assays, respectively. LC-ESI-MS/MS analysis revealed 12 components, with hesperidin being the most abundant (i.e., 1829.0001 μg/g). The ethanolic extract exhibited cytotoxic effects on cancer cell lines, with an IC50value of 0.959 mg/mL for dld-1, but with no effect on healthy cells. The bioavailability scores of the identified components support the in vitro findings and confirm the different assessed therapeutic effects. Keywords. |
| Anahtar Kelimeler |
| biological activity | flavonoids | LC-ESI-MS/MS | pharmacokinetics | Physalis acutifolia | polyphenols |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 7 |
| SCOPUS | 6 |
| Google Scholar | 7 |