Enterocin HZ produced by a wild Enterococcus faecium strain isolated from a traditional starter free pickled cheese
Yazarlar (5)
Niğde Üniversitesi, Türkiye
Prof. Dr. Hilal İŞLEROĞLU
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Doç. Dr. Kader TOKATLI
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
İnönü Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | JOURNAL OF DAIRY RESEARCH |
| Dergi ISSN | 0022-0299 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2014 |
| Cilt No | 81 |
| Sayı | 2 |
| Sayfalar | 164 / 172 |
| DOI Numarası | 10.1017/S0022029914000016 |
| Makale Linki | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/B44263FD0E76751BEA3BBEA5E226B24F/S0022029914000016a.pdf/enterocin-hz-produced-by-a-wild-enterococcus-faecium-strain-isolated-from-a-traditional-starter-free-pickled-cheese.pdf |
| Özet |
| Bacteriogenic Enterococcus faecium HZ was identified by using biochemical (Strep-API 20, API-50 CHL, fatty acid profile) and 16S rRNA analysis (99·99 %). Ent. faecium HZ was sensitive to clinically important antibiotics such as vancomycin, and did not have gelatinase and haemolysis activities. Enterocin HZ, a bacteriocin from Ent. faecium HZ, was sensitive to papain and tyripsin, but resistant to pepsin, lipase, catalase, α-amylase, organic solvents, detergents, ß-mercaptoethanol, and heat treatment (90 °C/30 min). It was biologically active at pH 2·0-9·0 and synthesised at the highest level in MRS or M17 broth at 32 or 37 °C with an inoculum amount of 0·1-0·5 % and an initial pH of 6·0-7·0. Enterocin HZ production reached maximum level at middle and late logarithmic phase and its molecular weight was ∼4·5 kDa. It was active against some Gram-positive foodborne bacteria. Ent. faecium HZ or its bacteriocin enterocin HZ is a good candidate to be studied as a food biopreservative since enterocin HZ showed strong bactericidal activity against Listeria monocytogenes in UHT milk and also Ent. faecium HZ grew very well in milk and produced enterocin HZ at maximum level. |
| Anahtar Kelimeler |
| Bacteriocin | Enterococcus faecium | enterocin HZ |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |