Non Ionic Contrast Media Induces Oxidative Stress and Apoptosis Through Ca2 Influx in Human Neutrophils
Yazarlar (6)
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
Alanya Alaaddin Keykubat Üniversitesi, Türkiye
Doç. Dr. Mehmet AYKUR
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Journal of Membrane Biology |
| Dergi ISSN | 0022-2631 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2012 |
| Cilt No | 245 |
| Sayı | 12 |
| Sayfalar | 833 / 840 |
| DOI Numarası | 10.1007/s00232-012-9491-x |
| Makale Linki | http://link.springer.com/10.1007/s00232-012-9491-x |
| Özet |
| Non-ionic contrast media (CM) can induce tissue kidney injury via activation of phagocytosis and oxidative stress, although the mechanisms of injury via neutrophils are not clear. We investigated the effects of CM on oxidative stress and Ca2+ concentrations in serum and neutrophils of humans. Ten migraine patients were used in the study. Serum and neutrophil samples from patients' peripheral blood were obtained before (control) and 30 min after non-ionic (iopromide) CM injection. The neutrophils were incubated with non specific transient receptor potential 2 (TRPM2) channel blocker, 2-aminoethoxydiphenyl borate (2-APB), and voltage gated Ca2+ channel blockers, verapamil plus diltiazem. Serum and neutrophil lipid peroxidation, apoptosis and intracellular Ca2+ concentrations levels were higher in the CM group than in controls. The neutrophilic reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) levels as well as serum vitamin E and β-carotene concentrations were lower in the CM group than in controls. Neutrophil lipid peroxidation levels were lower in the CM+2-APB and CM+verapamil-diltiazem groups than in the CM group, although GSH, GSH-Px and intracellular Ca2+ values increased in the CM+2-APB and CM+verapamil-diltiazem groups. However, caspase-3, caspase-9, vitamin A and vitamin C values were unaltered by CM treatment. In conclusion, we observed that CM induced oxidative stress and Ca2+ influx by decreasing vitamin E, β-carotene and Ca2+ release levels in human serum and neutrophils. However, we observed protective effects of Ca2+ channel blockers on Ca2+ influx in neutrophils. © Springer Science+Business Media, LLC 2012. |
| Anahtar Kelimeler |
| Ca release 2+ | Contrast media | Inflammation | Neutrophil | Oxidative stress |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 10 |
| SCOPUS | 11 |
| Google Scholar | 12 |