Molecular detection and genotyping of Acanthamoeba spp among stray dogs using conjunctival swab sampling       
Yazarlar (5)
Mehmet Karakuş
Sağlık Bilimleri Üniversitesi, Türkiye
Doç. Dr. Mehmet AYKUR Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Yusuf Özbel
Ege Üniversitesi, Türkiye
Seray Töz
Ege Üniversitesi, Türkiye
Hande Dağcı
Ege Üniversitesi, Türkiye
Makale Türü Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri)
Makale Alt Türü SCI, SSCI, AHCI, SCI-Exp dergilerinde yayınlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale
Dergi Adı Acta Tropica
Dergi ISSN 0001-706X Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 12-2016
Cilt No 164
Sayı 1
Sayfalar 23 / 26
DOI Numarası 10.1016/j.actatropica.2016.08.011
Makale Linki http://linkinghub.elsevier.com/retrieve/pii/S0001706X16304399
Özet
Acanthamoeba is one of the most common free-living amoebas (FLA) that present in environment. In humans, Acanthamoeba can cause an infection of the eye termed Acanthamoeba keratitis, which mostly occurs in contact lens wearers. In the present study, we aimed to screen the presence of Acanthamoeba DNA in stray dogs using previously collected conjunctival swab samples in a hyper-endemic area for canine leishmaniasis. Totally, 184 dogs were included in the study and 27 of them (14.6%) were found positive for Acanthamoeba according to the 18s rRNA gene sequencing. Two different genotypes (T4 and T5) were identified and T5 was firstly reported in Turkey in the present study. Statistical analysis was performed and no correlation was found between Leishmania and Acanthamoeba positivity (P < 0.05). To best of our knowledge, this is the first study conducted to screen Acanthamoeba among stray dogs. Further studies are necessary to reveal the infection status and genotypes among dogs and its possible correlation with leishmaniasis.
Anahtar Kelimeler
Acanthtamoeba | Conjunctival swab | Dog | Leishmania | Turkey