Partial characterisation of enterocin KP produced by Enterococcus faecalis KP a cheese isolate
Authors (5)
Prof. Dr. Hilal İŞLEROĞLU Tokat Gaziosmanpaşa Üniversitesi, Turkey
Prof. Dr. Zeliha Yıldırım Niğde Üniversitesi, Turkey
Asst. Prof. Dr. Mehmet TOKATLI Tokat Gaziosmanpaşa Üniversitesi, Turkey
Dr. Öğr. Üyesi Nilgün Öncül Gaziosmanpaşa Üniversitesi, Turkey
Metin Yıldırım Niğde Üniversitesi, Turkey
Article Type Original Article (Full articles published in SSCI, AHCI, SCI, SCI-Exp journals)
Journal Name INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY (Q3)
Journal ISSN 1364-727X Dergi Bilgileri (2012)
Indexes covering the journal SCI-Expanded
Article Language English Publication Date 02-2012
Kabul Tarihi – Publication Date 15-09-2011
Volume / Number / Page 65 / 1 / 90–97 DOI 10.1111/j.1471-0307.2011.00723.x
Article URL http://doi.wiley.com/10.1111/j.1471-0307.2011.00723.x
UAK Research Areas
Food Biotechnology
Summary
Enterococcus faecalis KP, a bacteriocin‐producing strain, was identified using 16S rRNA gene sequence homology (99%). Enterocin KP from E. faecalis KP was sensitive to papain and ß‐mercaptoethanol, but resistant to trypsin, pepsin, lipase, catalase, α‐amylase, organic solvents, detergents, EDTA and heat treatment (90°C/30 min). It was active at a wide pH range (2.0–8.0) and produced at maximum level in de Mann Rogosa Sharpe broth at 32°C with an inoculum amount of 0.1–1.0% and an initial pH of 5.5–7.5. It was active against some Gram‐positive bacteria, including Listeria monocytogenes. It showed bactericidal activity against L. monocytogenes and its molecular weight was approximately 5.8 kDa.
Keywords
Bacteriocin | Enterococcus faecalis KP | Enterocin KP
UN Sustainable Development Goals
Citations
Yöksis 10
Web of Science 12
Partial characterisation of enterocin KP produced by Enterococcus faecalis KP a cheese isolate