Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3a iR/i ,4 iS/i ,7 iR/i ,7a iS/i )-2-(4-(( iE/i )-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1 iH/i -4,7-methanoisoindole-1,3(2 iH/i )-dione derivatives on the enzyme activity/title
Yazarlar (9)
Türkiye
Cumhuriyet Üniversitesi, Türkiye
Bingöl Üniversitesi, Türkiye
Kırıkkale Üniversitesi, Türkiye
Prof. Dr. Meliha Burcu GÜRDERE
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Yakup BUDAK
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Mustafa CEYLAN
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
Bingöl Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY |
| Dergi ISSN | 1095-6670 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2018 |
| Cilt No | 32 |
| Sayı | 3 |
| Sayfalar | 22034 / 0 |
| DOI Numarası | 10.1002/jbt.22034 |
| Makale Linki | http://doi.wiley.com/10.1002/jbt.22034 |
| Özet |
| The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S-transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88-fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS-PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7methanoisoindole-1,3(2H)-dion derivatives (1a-g) were investigated on the enzyme activity. The inhibition parameters (IC and K values) were calculated for these compounds. IC values of these derivatives (1a-e) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. K values of these derivatives (1a-e) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found. |
| Anahtar Kelimeler |
| GST | Quail liver | enzyme purification | inhibition | methanoisoindole |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 20 |
| Google Scholar | 33 |