Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3a iR/i ,4 iS/i ,7 iR/i ,7a iS/i )-2-(4-(( iE/i )-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1 iH/i -4,7-methanoisoindole-1,3(2 iH/i )-dione derivatives on the enzyme activity/title
Yazarlar (9)
Prof. Dr. Yusuf Temel Türkiye
Prof. Dr. Ümit Muhammet Koçyiğit Cumhuriyet Üniversitesi, Türkiye
Dr. Öğr. Üyesi Mehmet Reşit Taysı Bingöl Üniversitesi, Türkiye
Prof. Dr. Faik Gökalp Kırıkkale Üniversitesi, Türkiye
Prof. Dr. Meliha Burcu GÜRDERE Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Yakup BUDAK Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Mustafa CEYLAN Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. İlhami Gülçin Atatürk Üniversitesi, Türkiye
Prof. Dr. Mehmet Çiftci Bingöl Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (Q4) | ||
| Dergi ISSN | 1095-6670 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2018 |
| Cilt / Sayı / Sayfa | 32 / 3 / 22034–0 | DOI | 10.1002/jbt.22034 |
| Makale Linki | http://doi.wiley.com/10.1002/jbt.22034 | ||
| UAK Araştırma Alanları |
Organik Kimya
|
||
| Özet |
| The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S‐transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88‐fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS‐PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)‐2‐(4‐((E)‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1H‐4,7methanoisoindole‐1,3(2H)‐dion derivatives (1a–g) were investigated on the enzyme activity. The inhibition parameters (IC50 and Ki values) were calculated for these compounds. IC50 values of these derivatives (1a–e) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. Ki values of these derivatives (1a–e … |
| Anahtar Kelimeler |
| GST | Quail liver | enzyme purification | inhibition | methanoisoindole |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 20 |
| Google Scholar | 34 |