Expression analysis and knockdown of two antennal odorant binding protein genes in Aedes aegypti
Yazarlar (2)
Doç. Dr. Meryem Şenay ŞENGÜL DEMİRAK
Virginia Polytechnic Institute and State University (Virginia Tech), Amerika Birleşik Devletleri
Virginia Polytechnic Institute and State University (Virginia Tech), Amerika Birleşik Devletleri
| Makale Türü |
Özgün Makale
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| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Journal of Insect Science |
| Dergi ISSN | 1536-2442 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2010 |
| Cilt No | 10 |
| Sayı | 1 |
| Sayfalar | 171 / 0 |
| DOI Numarası | 10.1673/031.010.14131 |
| Makale Linki | https://academic.oup.com/jinsectscience/article/10/1/171/884488 |
| Özet |
| The presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the host-seeking behavior of mosquitoes. It is important to establish a method in which a loss-of-function test can be performed to determine the possible role of these genes in olfaction. In this study, a double subgenomic Sindbis virus expression system was used to reduce the expression of two Obp genes in Aedes aegypti L (Diptera: Culicidae), AaegObp1 and AaegObp2. Quantitative real-time PCR analysis showed predominant expression of both genes in the female antennae, the primary olfactory tissue of mosquitoes.Moreover, at 11 days post virus-inoculation, the mRNA levels of AaegObp1 and AaegObp2 were significantly reduced in olfactory tissues of recombinant virus-inoculated ... |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 25 |