Characterization and expression of the odorant binding protein 7 gene in Anopheles stephensi and comparative analysis among five mosquito species
Yazarlar (2)
Doç. Dr. Meryem Şenay ŞENGÜL DEMİRAK
Virginia Polytechnic Institute and State University (Virginia Tech), Amerika Birleşik Devletleri
Virginia Polytechnic Institute and State University (Virginia Tech), Amerika Birleşik Devletleri
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Insect Molecular Biology |
| Dergi ISSN | 1365-2583 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2008 |
| Cilt No | 17 |
| Sayı | 6 |
| Sayfalar | 631 / 645 |
| Makale Linki | https://resjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2583.2008.00837.x |
| Özet |
| Odorant‐binding proteins (OBPs) are important molecular players in insect olfaction, which has a great influence on the host‐seeking behaviour of mosquitoes and other disease vectors. The mRNA level of the Anopheles gambiae Obp7 gene (Agam‐Obp7) is higher in the adult female antennae and is slightly reduced in the female heads after blood‐feeding. Here we report the cloning, sequencing, chromosomal mapping and transcript analysis of Aste‐Obp7, the Obp7 gene from the Asian malaria mosquito Anopheles stephensi. Quantitative reverse transcription PCR showed that in adult female mosquitoes, Aste‐Obp7 was expressed abundantly in the antennae, much less in pooled maxillary palp and proboscis and at the lowest level in the legs. The Aste‐Obp7 level in female antennae was significantly higher than in male antennae and it slightly increased 24 h after a bloodmeal. The same pattern held for leg ... |
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