Uncovering Phenotypic Diversity and DArTseq Marker Loci Associated with Antioxidant Activity in Common Bean
Yazarlar (14)
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
Sivas Bilim ve Teknoloji Üniversitesi, Türkiye
Prof. Dr. Onur SARAÇOĞLU
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Research Centre For Cereal And Industrial Crops, İtalya
Chinese University Of Hong Kong, Hong Kong
Çukurova Üniversitesi, Türkiye
Far Eastern Federal University, Rusya Federasyonu
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
University Of Karachi, Pakistan
Chonnam National University, Güney Kore
Chonnam National University, Güney Kore
Bolu Abant İzzet Baysal Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Genes |
| Dergi ISSN | 2073-4425 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2020 |
| Cilt No | 11 |
| Sayı | 1 |
| Sayfalar | 36 / 0 |
| DOI Numarası | 10.3390/genes11010036 |
| Makale Linki | https://www.mdpi.com/2073-4425/11/1/36 |
| Özet |
| Antioxidants play an important role in animal and plant life owing to their involvement in complex metabolic and signaling mechanisms, hence uncovering the genetic basis associated with antioxidant activity is very important for the development of improved varieties. Here, a total of 182 common bean (Phaseolus vulgaris) landraces and six commercial cultivars collected from 19 provinces of Turkey were evaluated for seed antioxidant activity under four environments and two locations. Antioxidant activity was measured using ABTS radical scavenging capacity and mean antioxidant activity in common bean landraces was 20.03 µmol TE/g. Analysis of variance reflected that genotype by environment interaction was statistically non-significant and heritability analysis showed higher heritability of antioxidant activity. Variations in seed color were observed, and a higher antioxidant activity was present in seeds having colored seed as compared to those having white seeds. A negative correlation was found between white-colored seeds and antioxidant activity. A total of 7900 DArTseq markers were used to explore the population structure that grouped the studied germplasm into two sub-populations on the basis of their geographical origins and trolox equivalent antioxidant capacity contents. Mean linkage disequilibrium (LD) was 54%, and mean LD decay was 1.15 Mb. Mixed linear model i.e., the Q + K model demonstrated that four DArTseq markers had significant association (p < 0.01) for antioxidant activity. Three of these markers were present on chromosome Pv07, while the fourth marker was located on chromosome Pv03. Among the identified markers, DArT-3369938 marker showed maximum (14.61%) variation. A total of four putative candidate genes were predicted from sequences reflecting homology to identified DArTseq markers. This is a pioneering study involving the identification of association for antioxidant activity in common bean seeds. We envisage that this study will be very helpful for global common bean breeding community in order to develop cultivars with higher antioxidant activity. |
| Anahtar Kelimeler |
| Genetic basis | Genotype by environment interaction | Germplasm characterization | GWAS | Mixed linear model | Phaseolus vulgaris |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 28 |
| SCOPUS | 28 |
| Google Scholar | 40 |