Purification and determination of inhibitory activity of recombinant soyacystatin for surimi application
Yazarlar (2)
Prof. Dr. Özlem AKPINAR
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
University Of Southern California, Amerika Birleşik Devletleri
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Molecular Nutrition and Food Research |
| Dergi ISSN | 1613-4125 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2005 |
| Cilt No | 49 |
| Sayı | 3 |
| Sayfalar | 247 / 255 |
| DOI Numarası | 10.1002/mnfr.200400059 |
| Makale Linki | http://doi.wiley.com/10.1002/mnfr.200400059 |
| Özet |
| A recombinant soyacystatin (r-soyacystatin) was tested for its inhibitory activity against cysteine proteinase of Pacific whiting and its activity was compared to that of egg white cystatin. A recombinant soyacystatin expressed in Escherichia coli was purified to electrophoretic homogeneity using phenylSepharose and DEAE-Sepharose. Native egg white cystatin was purified by using affinity chromatography on CM-papain-Sepharose generated in our lab. Egg white cystatin and soyacystatin were tested for proteinase inhibitory activity against commercial papain and also cathepsin L purified from Pacific whiting muscle. The r-soyacystatin exhibited papain-like protease inhibition activity comparable to that of the egg white cystatin, which could inhibit papain and Pacific whiting cathepsin L. The r-soyacystatin subsequently inhibited the autolytic activity of Pacific whiting surimi. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Anahtar Kelimeler |
| Autolytic activity | Egg white cystatin | Pacific whiting | Soyacystatin | Surimi |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 4 |
| SCOPUS | 4 |