Comparison of acid and enzymatic hydrolysis of tobacco stalk xylan for preparation of xylooligosaccharides    
Yazarlar (4)
Prof. Dr. Özlem AKPINAR Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Kader Erdoğan
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ufuk Bakır
Middle East Technical University (Metu), Türkiye
Levent Yılmaz
Orta Doğu Teknik Üniversitesi, Türkiye
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Lwt
Dergi ISSN 0023-6438 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2010
Cilt No 43
Sayı 1
Sayfalar 119 / 125
DOI Numarası 10.1016/j.lwt.2009.06.025
Makale Linki http://linkinghub.elsevier.com/retrieve/pii/S0023643809001984
Özet
Tobacco stalk (TS), a major agricultural waste in the Black Sea region of Turkey, was used for the production of xylooligosaccharides (XOs). It contains about 22 g/100 g xylan whose composition was determined as 93.5 g/100 g xylose, 6.54 g/100 g glucose and 11.2 g/100 g uronic acid after complete acid hydrolysis. XO production was performed by enzymatic and acid hydrolysis of xylan which was obtained by alkali extraction from tobacco stalk. In enzyme hydrolysis, xylan was hydrolyzed using a xylanase preparation and the effects of pH, temperature, hydrolysis period, substrate and enzyme concentrations on the xylooligosaccharide yield and degree of polymerization were investigated. For enzymatic hydrolysis under optimum conditions XO yield with respect to tobacco stalk xylan (TSX) was 8.2 g/100 g after 8 h and 11.4 g/100 g after 24 h reaction period. In the acid hydrolysis, sulfuric acid was used and the hydrolyzate contained different amount of oligosaccharides and monosaccharides. For acid hydrolysis under optimum conditions, XO yield with respect to TSX was 13.0 g/100 g. Enzymatically obtained oligosaccharides were purified via ultrafiltration by using 10 and 3 kDa membranes. After a two-step membrane processing, the permeate containing mostly oligosaccharides was obtained. © 2009 Elsevier Ltd. All rights reserved.
Anahtar Kelimeler
Lignocellulose | Prebiotics | Tobacco stalk | Xylooligosaccharides