Preparation of cellooligosaccharides Comparative study
Yazarlar (2)
Prof. Dr. Özlem AKPINAR
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Oregon State University, Amerika Birleşik Devletleri
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Journal of Food Agriculture and Environment |
| Dergi ISSN | 1459-0255 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2008 |
| Cilt No | 6 |
| Sayı | 1 |
| Sayfalar | 55 / 61 |
| Özet |
| Cellooligosaccharides having a specific degree of polymerization are commonly used in mechanistic studies of cellulolytic enzymes and since they are not hydrolyzed or absorbed by the human gastrointestinal tract, (non-digestible oligosaccharides) they may have potential as non-caloric bulking agents in food products. Considering the heavy demand for cellooligosaccharides in many cellulase/cellulose laboratories, many methods were developed for the separation of cellooligosaccharides. They are typically produced by the controlled hydrolysis of cellulose, which produces a mixture of cellooligosaccharides differing with respect to their degree of polymerization, followed by chromatographic fractionation to obtain preparations homogeneous with respect to degree of polymerization. This research summarizes a comparative study of suggested fractionation methods. Charcoal-celite adsorption, molecular exclusion and ion-mediated silica and cellulose affinity/partition chromatography were evaluated. Adsorption chromatography with charcoal-celite stationary phases was the most generally applicable method for the preparation of near gram quantities of pure cellooligosaccharides in the DP range of three to seven. Cellulose affinity/partition chromatography was found to be the least time consuming and most economical method for the preparation of cellotetraose and cellopentaose. Ion-mediated chromatography was the most readily applicable for analytical applications. |
| Anahtar Kelimeler |
| Cellodextrin | Cellooligosaccharides | Cellulose | Fractionation | Prebiotics |
