Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity

Temel, Yusuf; Koçyiğit, Ümit Muhammet; Taysı, M Şerif; Gökalp, Faik; GÜRDERE, MELİHA BURCU; BUDAK, YAKUP; CEYLAN, MUSTAFA; Gülçin, İlhami; Çiftçi, Mehmet

doi:10.1002/jbt.22034

Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity

Yazarlar (9)

Yusuf Temel
Bingöl Üniversitesi, Türkiye

Ümit Muhammet Koçyiğit
Sivas Cumhuriyet Üniversitesi, Türkiye

M Şerif Taysı

Faik Gökalp
Kırıkkale Üniversitesi, Türkiye

Prof. Dr. Meliha Burcu GÜRDERE Tokat Gaziosmanpaşa Üniversitesi, Türkiye

Prof. Dr. Yakup BUDAK Tokat Gaziosmanpaşa Üniversitesi, Türkiye

Prof. Dr. Mustafa CEYLAN Tokat Gaziosmanpaşa Üniversitesi, Türkiye

İlhami Gülçin
Atatürk Üniversitesi, Türkiye

Mehmet Çiftçi
Fırat Üniversitesi, Türkiye

Makale Türü	Özgün Makale
Makale Alt Türü	SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı	JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Dergi ISSN	1095-6670 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Dergi Grubu	Q4
Makale Dili	İngilizce
Basım Tarihi	03-2018
Cilt No	32
Sayı	3
Sayfalar	22034 / 0
DOI Numarası	10.1002/jbt.22034
Makale Linki	http://doi.wiley.com/10.1002/jbt.22034

Özet

The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S-transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88-fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS-PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7methanoisoindole-1,3(2H)-dion derivatives (1a-g) were investigated on the enzyme activity. The inhibition parameters (IC and K values) were calculated for these compounds. IC values of these derivatives (1a-e) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. K values of these derivatives (1a-e) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found.

Anahtar Kelimeler

GST | Quail liver | enzyme purification | inhibition | methanoisoindole

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
WoS	20
Google Scholar	33

Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity

Dergi Adı	JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Yayıncı	John Wiley & Sons Inc.
Açık Erişim	Hayır
ISSN	1095-6670
E-ISSN	1099-0461
CiteScore	6,0
SJR	0,772
SNIP	0,725

Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity

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