Tumor Necrosıs Factor Alpha Beta and Interferon Gamma Gene Polymorphisms in Turkish Breast Cancer Patients
Yazarlar (5)
Doç. Dr. Nevin KARAKUŞ
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
Research Hospital, Türkiye
Ondokuz Mayis Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | DNA and Cell Biology |
| Dergi ISSN | 1044-5498 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2011 |
| Cilt No | 30 |
| Sayı | 6 |
| Sayfalar | 371 / 377 |
| DOI Numarası | 10.1089/dna.2010.1113 |
| Özet |
| Cytokine genes are important for researching cancer predisposition to cancers that elicit anti-tumor immune response. In this study, we investigated the association between breast cancer and tumor necrosis factor alpha (TNF-α) -308 (G>A), TNF-β +252 (A>G), and interferon gamma (IFN-γ) +874 (T>A) gene polymorphisms in a Turkish population. This study involved 204 female breast cancer patients and 204 healthy female controls. Genomic DNA was extracted from EDTA-preserved peripheral venous blood of patients and controls by a salting-out method and analyzed by polymerase chain reaction, allele-specific oligonucleotide polymerase chain reaction, and restriction fragment length polymorphism. TNF-α -308 genotype was found to have no effect on breast cancer susceptibility. However, there were statistically significant differences between the genotype frequencies of patients and controls for TNF-β polymorphism (p=0.016) and the allele and genotype frequencies for the IFN-γ polymorphism (p=0.0312 and p0.001, respectively). In the composite genotype analysis, the TNF-α/β GAAG composite genotype (p=0.0424), the TNF-α/IFN-γ GGTT and GATT composite genotypes (p=0.0296 and p=0.0129, respectively), the TNF-β/IFN-γ AGTT composite genotype (p=0.0003), and the TNF-α/β/IFN-γ GGAGTT and GAAGTT composite genotypes (p=0.0437 and p=0.0038, respectively) were estimated to have a protective effect against breast cancer. However, the TNF-α/IFN-γ GGTA composite genotype is a risk factor for breast cancer (p=0.0156). In conclusion, TNF-β +252GG genotype was found more frequent in Turkish breast cancer patients than controls and IFN-γ TA+AA genotypes were estimated to increase breast cancer risk significantly in Turkish population. © 2011 Mary Ann Liebert, Inc. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 18 |
| SCOPUS | 23 |
| Google Scholar | 34 |