Formulation of Hesperidin‐loaded In situ Gel for Ocular Drug Delivery: A Comprehensive Study
Yazarlar (12)
Erzincan Binali Yıldırım Üniversitesi, Türkiye
T.C. Sağlık Bakanlığı,, Türkiye
Selçuk Üniversitesi, Türkiye
Erciyes Üniversitesi, Türkiye
Süleyman Demirel Üniversitesi, Türkiye
Erciyes Üniversitesi, Türkiye
Hacettepe Üniversitesi, Türkiye
Erzincan Binali Yıldırım Üniversitesi, Türkiye
Erzincan Binali Yıldırım Üniversitesi, Türkiye
Hacettepe Üniversitesi, Türkiye
Dr. Öğr. Üyesi Nihat KURT
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Journal of the Science of Food and Agriculture |
| Dergi ISSN | 0022-5142 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | Türkçe |
| Basım Tarihi | 03-2024 |
| Cilt No | 104 |
| Sayı | 10 |
| Sayfalar | 5846 / 5859 |
| DOI Numarası | 10.1002/jsfa.13407 |
| Makale Linki | http://dx.doi.org/10.1002/jsfa.13407 |
| Özet |
| BACKGROUND: Allergic conjunctivitis is one of the most common eye disorders. Different drugs are used for its treatment. Hesperidin is an active substance isolated from Citrus sinensis L. (Rutaceae) fruit peels, with known anti-inflammatory activity but low solubility. It was complexed with cyclodextrin and encapsulated in situ gel to extend its duration in the eye. RESULTS: The optimized formulation comprised 1% hesperidin, 1.5% hydroxyethyl cellulose, and 16% poloxamer 407. The viscosity at 25 °C was 492 ± 82 cP, and at 35 °C it was 8875 ± 248 cP, the pH was 7.01 ± 0.03, gelation temperature was 34 ± 1.3 °C, and gelation time was 33 ± 1.2 s. There was a 66% in vitro release in the initial 2 h, with a burst effect. A lipoxygenase (LOX) inhibition test determined that hesperidin was active at high doses on leukotyrens seen in the body in allergic diseases. In cell-culture studies, the hesperidin cyclodextrin complex loaded in situ gel, BRN9-CD (poloxamer 16%, hydroxy ethyl cellulose (HEC) 1.5%), enhanced cell viability in comparison with the hesperidin solution. It was determined that BRN9-CD did not cause any irritation in the ocular tissues in the Draize test. CONCLUSION: The findings of this study demonstrate the potential of the in situ gel formulation of hesperidin in terms of ease of application and residence time on the ocular surface. Due to its notable LOX inhibition activity and positive outcomes in the in vivo Draize test, it appears promising for incorporation into pharmaceutical formulations. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. |
| Anahtar Kelimeler |
| cell viability | cyclodextrin | Draize test | hesperidin | lox inhibition |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 13 |
| SCOPUS | 16 |
| Google Scholar | 18 |