Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities
Yazarlar (7)
Prof. Dr. Ramazan ERENLER
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Artvin Çoruh Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | PHARMACEUTICAL BIOLOGY |
| Dergi ISSN | 1388-0209 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2017 |
| Cilt No | 55 |
| Sayı | 1 |
| Sayfalar | 1646 / 1653 |
| DOI Numarası | 10.1080/13880209.2017.1310906 |
| Özet |
| Origanum (Lamiaceae) has been used in food and pharmaceutical industries. Isolation and identification of bioactive compounds from Origanum rotundifolium Boiss. and investigation of their antiproliferative and antioxidant activities. The aerial part of O. rotundifolium was dried and powdered (1.0 kg ±2.0 g) then extracted with hexane, ethyl acetate, methanol and water. Solvent (3 × 1 L) was used for each extraction for a week at room temperature. The aqueous extract was partitioned with ethyl acetate (3 × 1 L) to yield the water/EtOAc extract subjected to chromatography to isolate the active compounds. The structures of isolated compounds were elucidated by 1 D, 2 D NMR and LC-TOF/MS. Apigenin (1), ferulic acid (2), vitexin (3), caprolactam (4), rosmarinic acid (5), and globoidnan A (6) were isolated and identified. Globoidnan A (6), vitexin (3), and rosmarinic acid (5) revealed the excellent DPPH scavenging effect with IC values of 22.4, 31.4, 47.2 μM, respectively. Vitexin (3) (IC 3.6), globoidnan A (6) (IC 4.6), apigenin (1) (IC 8.9) and ferulic acid (2) exhibited more ABTS activity than standard Trolox (IC 13.8 μg/mL). Vitexin (3) revealed the most antiproliferative activity against HeLa, HT29, C6 and Vero cells lines with IC values of 35.6, 32.5, 41.6, 46.7 (μM), respectively. Globoidnan A (6) has the most antioxidant effects on all assays. This has to do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent. |
| Anahtar Kelimeler |
| Secondary metabolites | isolation | chromatography | spectroscopy |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 67 |