COMPARISON OF PHYTOCHEMICALS AND ANTIOXIDANT CAPACITY OF HYPERICUM PERFORATUM; WILD PLANT PARTS AND IN VITRO SAMPLES
Yazarlar (4)
C. Yaman Yozgat Bozok University, Türkiye
Önlü Muş Alparslan Üniversitesi, Türkiye
H. A.A. Ahmed Ankara Üniversitesi, Türkiye
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Journal of Animal and Plant Sciences (Q3)
Dergi ISSN 1018-7081 Dergi Bilgileri (2022)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 04-2022
Kabul Tarihi – Yayınlanma Tarihi 13-08-2021
Cilt / Sayı / Sayfa 32 / 2 / 596–603 DOI 10.36899/JAPS.2022.2.0459
Makale Linki http://dx.doi.org/10.36899/japs.2022.2.0459
UAK Araştırma Alanları
Organik Kimya Spektroskopi Nanoteknoloji
Özet
The aim of study is to compare the phytochemicals and free radical scavenging activities in methanol extracts of flower, leaf, stem in vitro plantlet, callus of Hypericum perforatum L. In vitro cultures of H. perforatum was established by using MS-B5 medium contained plant growth regulators such as BAP, TDZ and picloram. Total phenolics and flavonoids were analysed by spectrophotometric methods.The stem was the richest in total phenolics (228.9 mg GAE/g extract) and flavonoids (102.4 mg QE/g extract). Quinic acid, gallic acid, (+)-catechin, ferulic acid, vanillic acid, p-coumaric acid, caffeic acid, and quercetin were determinated by LC-MS/MS. Free radical scavenging activities (ABTS and DPPH) of all samples were detected as IC50 values, and was compared to standards such as trolox and ascorbic acid. As a result, the stem exhibited the stronger antioxidant activities than other samples, and vanillic acid, ferulic acid and gallic acid could be produced by in vitro culture.
Anahtar Kelimeler
antioxidant activity | Hypericum perforatum | in vitro plantlets | phytochemicals
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Web of Science 16
Scopus 18
Google Scholar 31
COMPARISON OF PHYTOCHEMICALS AND ANTIOXIDANT CAPACITY OF HYPERICUM PERFORATUM; WILD PLANT PARTS AND IN VITRO SAMPLES