Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum
Yazarlar (2)
Tünay Karan Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
|
||
| Dergi Adı | International Journal of Secondary Metabolite | ||
| Dergi ISSN | 2148-6905 | ||
| Dergi Tarandığı Indeksler | TR DİZİN | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2017 |
| Cilt / Sayı / Sayfa | 4 / 3 / 16–19 | DOI | – |
| UAK Araştırma Alanları |
Organik Kimya
|
||
| Özet |
| Cyanobacterialmetabolites are natural products that have an important feature inpharmaceutical and medicinal industries. In this study, the presence of thesecondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler)Anagnostidis isolated from Tokat Yesilirmak River and its production in saltstress and pH stress was investigated. In salt stress experiments,cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium inerlenmayers of 0.5, 1.0, 3.0, 5.0 M. pH stresswas executed at 5 and 9. Norharmane amount was determined by HPLC usingC18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml /min. The amount of norharman metabolite (μg/g) was calculated according to theGauss method by drawing a calibration curve over the absorbance value of thestandard 247 nm wavelength. According to the analysis results, metaboliteproduction was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M,there was no norharmane production. Thenorharmane production is higher at pH 5 (1.293 μg /g) than that of the pH 9 (0.448 μg /g). |
| Anahtar Kelimeler |
