Studies on the antioxidant potential of flavones of Allium vineale isolated from its water soluble fraction     
Yazarlar (4)
İbrahim Demirtaş
Çankırı Karatekin Üniversitesi, Türkiye
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Mahfuz Elmastaş
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ahmet Göktaşoğlu
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Food Chemistry
Dergi ISSN 0308-8146 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2013
Cilt No 136
Sayı 1
Sayfalar 34 / 40
DOI Numarası 10.1016/j.foodchem.2012.07.086
Makale Linki http://linkinghub.elsevier.com/retrieve/pii/S0308814612012010
Özet
The aim of this work was to examine the chemical constituents and antioxidant potential of water-soluble fractions from the commonly consumed vegetable, Allium vineale. The water-soluble fraction, containing phenolic compounds, was extracted with ethyl acetate to obtain flavonoids which were separated and purified by repeated column chromatography over Sephadex LH-20, RP C18 and silica gel. The isolated compounds were identified according to their physicochemical properties and spectral data (UV, HPLC-TOF/MS, (1)H NMR, (13)C NMR and 2D NMR). Three flavonoids were isolated and identified as chrysoeriol-7-O-[2″-O-E-feruloyl]-β-d-glucoside (1), chrysoeriol (2), and isorhamnetin-3-β-d-glucoside (3). Antioxidant studies of the aqueous extract and three isolated compounds, 1, 2, 3, were undertaken and they were found to have significant antioxidant activity. Antioxidant activities were evaluated for total antioxidant activity by the ferric thiocyanate method, ferric ion (Fe(3+)) reducing antioxidant power assay (FRAP), ferrous ion (Fe(2+)) metal chelating activity, and DPPH free radical-scavenging activity. The water-soluble ethyl acetate and methanol extraction methods were also compared using HPLC-TOF/MS.
Anahtar Kelimeler
Allium vineale | Water-soluble flavonoid extraction | Antioxidant activity | Flavonoids