In vitro antiproliferative cytotoxic activity of 2 3 biindole againstvarious cancer cell lines
Yazarlar (4)
Salih Ökten Kırıkkale Üniversitesi, Türkiye
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Tuğba Kul Köprülü Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Şaban Tekin Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | TURKISH JOURNAL OF BIOLOGY (Q4) | ||
| Dergi ISSN | 1300-0152 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2015 |
| Cilt / Sayı / Sayfa | 39 / 1 / 15–22 | DOI | 10.3906/biy-1402-60 |
| Makale Linki | http://online.journals.tubitak.gov.tr/openDoiPdf.htm?mKodu=biy-1402-60 | ||
| Özet |
| 2,3\\\'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3\\\'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action. |
| Anahtar Kelimeler |
| 2,3 '-Biindole | anticancer | cytotoxicity | apoptosis | antitopoisomerase | C6 cell line | HeLa cell line | HT29 cell line |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 15 |