Serologic and molecular determination and phylogenetic analysis of some viruses infecting pepper in Turkey
   
Yazarlar (1)
Doç. Dr. Şerife TOPKAYA Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Emirates Journal of Food and Agriculture (Q4)
Dergi ISSN 2079-052X Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 11-2022
Cilt / Sayı / Sayfa 34 / 11 / 923–930 DOI 10.9755/ejfa.2022.v34.i11.2961
Makale Linki https://ejfa.me/index.php/journal/article/view/2961
Özet
Viruses are among the major agents causing yield loss in pepper production. During the 2016-2017, a survey was performed in pepper fields to determine of some viruses in pepper plants showing virus-like symptoms in different provinces of Turkey. Plant samples were assayed using DAS-ELISA with specific antisera for cucumber mosaic virus (CMV), tomato spotted wilt virus (TSWV), tomato yellow leaf curl virus (TYLCV), alfalfa mosaic virus (AMV), and pepper mild mottle virus (PMMoV). The DAS-ELISA results indicated that 42.2, 17.5, 17.5, and 10.6 of the 421 samples were infected with CMV, TSWV, AMV, and PMMoV, respectively. The positive samples were further analysed by RT-PCR and sequencing. Based on sequence analyses, a phylogenetic tree was drawn for each of CMV, PMMoV and AMV. Phylogenetic analysis showed that CMV isolates were placed in subgroup IA within the major group I. The …
Anahtar Kelimeler
Alfalfa mosaic virus | Cucumber mosaic virus | Pepper | Pepper mild mottle virus