Molecular Analysis of the Global Population of Potato Virus S Redefines Its Phylogeny, and Has Crop Biosecurity Implications
Yazarlar (4)
Doç. Dr. Şerife TOPKAYA Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ali Çelik Bolu Abant İzzet Baysal Üniversitesi, Türkiye
Adyatma Irawan Santosa
Universitas Gadjah Mada, Endonezya
Universitas Gadjah Mada, Endonezya
Roger A.C. Jones
The University Of Western Australia, Avustralya
The University Of Western Australia, Avustralya
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Viruses (Q2) | ||
| Dergi ISSN | 1999-4915 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2023 |
| Cilt / Sayı / Sayfa | 15 / 5 / 1104–0 | DOI | 10.3390/v15051104 |
| Makale Linki | https://www.mdpi.com/1999-4915/15/5/1104 | ||
| Özet |
| In 2020, 264 samples were collected from potato fields in the Turkish provinces of Bolu, Afyon, Kayseri and Niğde. RT-PCR tests, with primers which amplified its coat protein (CP), detected potato virus S (PVS) in 35 samples. Complete CP sequences were obtained from 14 samples. Phylogenetic analysis using non-recombinant sequences of (i) the 14 CP’s, another 8 from Tokat province and 73 others from GenBank; and (ii) 130 complete ORF, RdRp and TGB sequences from GenBank, found that they fitted within phylogroups, PVSI, PVSII or PVSIII. All Turkish CP sequences were in PVSI, clustering within five subclades. Subclades 1 and 4 were in three to four provinces, whereas 2, 3 and 5 were in one province each. All four genome regions were under strong negative selection constraints (ω = 0.0603–0.1825). Considerable genetic variation existed amongst PVSI and PVSII isolates. Three neutrality test methods showed PVSIII remained balanced whilst PVSI and PVSII underwent population expansion. The high fixation index values assigned to all PVSI, PVSII and PVSIII comparisons supported subdivision into three phylogroups. As it spreads more readily by aphid and contact transmission, and may elicit more severe symptoms in potato, PVSII spread constitutes a biosecurity threat for countries still free from it. |
| Anahtar Kelimeler |
| biosecurity | gene flow | gene transcript analysis | genetic diversity | incursions | molecular characterization | phylogeny | population genetics | potato | PVS | recombination | Turkish provinces |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 16 |
| SCOPUS | 16 |
| Google Scholar | 24 |