Investigation of plasmid mediated AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae isolates by phenotypic and genotypic.
Yazarlar (6)
Düzce Üniversitesi, Türkiye
Prof. Dr. Umut Safiye ŞAY COŞKUN
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Düzce Üniversitesi, Türkiye
İstanbul Medeniyet Üniversitesi, Türkiye
Düzce Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | The Journal of the Pakistan Medical Association |
| Dergi ISSN | 0030-9982 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2019 |
| Cilt No | 69 |
| Sayı | 6 |
| Sayfalar | 834 / 839 |
| Özet |
| To investigate the susceptibility and specificity of the phenotypic methods to determine plasmidmediated AmpC. The cross-sectional study was conducted at Duzce University Faculty of Medicine, Microbiology Laboratory from January 2015 to June 2016, and comprised Escherichia coli and Klebsiella pneumonia isolates intermediate susceptible or resistant to cefoxitine. Combined disk diffusion test, double disc synergy test, agar gradient test and polymerase chain reaction were used to detect plasmid-mediated AmpC. Of the 2024 E. coli samples, 44(2.17%), and of the 792 K. pneumoniae samples, 16(2%) were included. Combined disk diffusion test had susceptibility of 68% and specificity of 50%; double disc synergy test 24% and 82%; and agar gradient test 40% and 68%. Of the isolates positively detected by polymerase chain reaction method, more than one gene region positivity was detected in 15(25%) isolates. All three phenotypic methods were found to be insufficient to detect plasmid-mediated AmpC positivity. |
| Anahtar Kelimeler |
| Plasmid-mediated AmpC beta-lactamases | PCR | Escherichia coli | Klebsiella pneumoniae |
