Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol
Yazarlar (9)
Lokman Durmaz Erzincan Binali Yıldırım Üniversitesi, Türkiye
Adem Ertürk Atatürk Üniversitesi, Türkiye
Mehmet Akyüz Kilis 7 Aralik Üniversitesi, Türkiye
Leyla Polat Köse T.C. Beykent Üniversitesi, Türkiye
Eda Mehtap Uç
Atatürk Üniversitesi, Türkiye
Atatürk Üniversitesi, Türkiye
Dr. Öğr. Üyesi Zeynebe BİNGÖL Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Rüya Sağlamtaş Ağrı İbrahim Çeçen Üniversitesi, Türkiye
Saleh Alwasel
College Of Sciences, Suudi Arabistan
College Of Sciences, Suudi Arabistan
İlhami Gülçin Atatürk Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Molecules (Q4) | ||
| Dergi ISSN | 1420-3049 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2022 |
| Cilt / Sayı / Sayfa | 27 / 10 / – | DOI | 10.3390/molecules27103091 |
| Makale Linki | http://dx.doi.org/10.3390/molecules27103091 | ||
| Özet |
| Coumestrol (3,9-dihydroxy-6-benzofuran [3,2-c] chromenone) as a phytoestrogen and polyphenolic compound is a member of the Coumestans family and is quite common in plants. In this study, antiglaucoma, antidiabetic, anticholinergic, and antioxidant effects of Coumestrol were evaluated and compared with standards. To determine the antioxidant activity of coumestrol, several methods—namely N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD•+)-scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS•+)-scavenging activity, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•)-scavenging activity, potassium ferric cyanide reduction ability, and cupric ion (Cu2+)-reducing activity—were performed. Butylated hydroxyanisole (BHA), Trolox, α-Tocopherol, and butylated hydroxytoluene (BHT) were used as the reference antioxidants for comparison. Coumestrol scavenged the DPPH radical with an IC50 value of 25.95 µg/mL (r2: 0.9005) while BHA, BHT, Trolox, and α-Tocopherol demonstrated IC50 values of 10.10, 25.95, 7.059, and 11.31 µg/mL, respectively. Evaluating these results, Coumestrol had a similar DPPH•-scavenging effect to BHA and Trolox, better than BHT and α-Tocopherol. In addition, the inhibition effects of Coumestrol were tested against the metabolic enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II), and α-glycosidase, which are associated with some global diseases such as Alzheimer’s disease (AD), glaucoma, and diabetes. Coumestrol exhibited Ki values of 10.25 ± 1.94, 5.99 ± 1.79, 25.41 ± 1.10, and 30.56 ± 3.36 nM towards these enzymes, respectively. |
| Anahtar Kelimeler |
| acetylcholinesterase | antioxidant activity | butyrylcholinesterase | carbonic anhydrase | coumestrol | phenolic compound | α-glycosidase |