Apoptosis Induced by Tarantula cubensis Crude Venom (Theranekron® D6) in Cancer Cells
Yazarlar (3)
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Alanya Alaaddin Keykubat Üniversitesi, Türkiye
Doç. Dr. Aykut ÖZGÜR
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Revista Brasileira De Farmacognosia |
| Dergi ISSN | 1981-528X Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | Türkçe |
| Basım Tarihi | 12-2021 |
| Cilt No | 31 |
| Sayı | 6 |
| Sayfalar | 824 / 831 |
| DOI Numarası | 10.1007/s43450-021-00221-x |
| Makale Linki | https://link.springer.com/article/10.1007/s43450-021-00221-x |
| Özet |
| Natural compounds from venomous animals have a big potent in cancer drug discovery. This study aimed to investigate the apoptotic potential of Theranekron D6, an alcoholic extract of the venom from the whole spider Tarantula cubensis, in cells derived from breast (MCF-7), lung (A549), and bone (Saos-2) cancer. TD6 stimulated a significant increase in the oxidative stress and stimulated apoptosis in cancer cells. To understand the molecular mechanism for the induction of cell death by TD6, fluorometric apoptosis/necrosis and RT-PCR assays were performed. In MCF-7 cell line, TD6 increased CAS-9 expression, as well as the mRNA and protein expression ratio of BAX/BCL-2. Furthermore, protein expression level of the Pro-CAS-9 was decreased in TD6-treated MCF-7 cells. TD6 increased CAS-9 and CAS-3 expressions in A549 and Saos-2 cell lines, but the expression ratio of the BAX/BCL-2 did not increase at the gene transcription level in these cancer cell lines. Moreover, the protein level of BAX/BCL-2 ratio was increased in TD6-treated Saos-2 cells, and the Pro-CAS-3 and Pro-CAS-9 were down-regulated in Saos-2 and A549 cell lines. Therefore, Theranekron D6 could be an excellent alternative therapeutic drug for the treatment of malignant neoplasm as an apoptosis inducer agent. Graphical abstract: [Figure not available: see fulltext.]. |
| Anahtar Kelimeler |
| Cancer | Caspase cascade pathway | Chemotherapy | Cytotoxicity | Total oxidant capacity | Total oxidant status |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| SCOPUS | 15 |
| Google Scholar | 20 |