Proteomic profiling of HBV infected liver biopsies with different fibrotic stages
Yazarlar (11)
İstanbul Teknik Üniversitesi, Türkiye
Umraniye Teaching And Research Hospital, Türkiye
Prof. Dr. Abdurahman ŞAHİN
Umraniye Teaching And Research Hospital, Türkiye
Umraniye Teaching And Research Hospital, Türkiye
Umraniye Teaching And Research Hospital, Türkiye
Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
İstanbul Teknik Üniversitesi, Türkiye
Umraniye Teaching And Research Hospital, Türkiye
Umraniye Teaching And Research Hospital, Türkiye
İstanbul Teknik Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Proteome Science |
| Dergi ISSN | 1477-5956 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2017 |
| Cilt No | 15 |
| Sayı | 1 |
| Sayfalar | 1 / 11 |
| DOI Numarası | 10.1186/s12953-017-0114-4 |
| Makale Linki | http://proteomesci.biomedcentral.com/articles/10.1186/s12953-017-0114-4 |
| Özet |
| Background: Hepatitis B virus (HBV) is a global health problem, and infected patients if left untreated may develop cirrhosis and eventually hepatocellular carcinoma. This study aims to enlighten pathways associated with HBV related liver fibrosis for delineation of potential new therapeutic targets and biomarkers. Methods: Tissue samples from 47 HBV infected patients with different fibrotic stages (F1 to F6) were enrolled for 2D-DIGE proteomic screening. Differentially expressed proteins were identified by mass spectrometry and verified by western blotting. Functional proteomic associations were analyzed by EnrichNet application. Results: Fibrotic stage variations were observed for apolipoprotein A1 (APOA1), pyruvate kinase PKM (KPYM), glyceraldehyde 3-phospahate dehydrogenase (GAPDH), glutamate dehydrogenase (DHE3), aldehyde dehydrogenase (ALDH2), alcohol dehydrogenase (ALDH1A1), transferrin (TRFE), peroxiredoxin 3 (PRDX3), phenazine biosynthesis-like domain-containing protein (PBLD), immuglobulin kappa chain C region (IGKC), annexin A4 (ANXA4), keratin 5 (KRT5). Enrichment analysis with Reactome and Kegg databases highlighted the possible involvement of platelet release, glycolysis and HDL mediated lipid transport pathways. Moreover, string analysis revealed that HIF-1α (Hypoxia-inducible factor 1-alpha), one of the interacting partners of HBx (Hepatitis B X protein), may play a role in the altered glycolytic response and oxidative stress observed in liver fibrosis. Conclusions: To our knowledge, this is the first protomic research that studies HBV infected fibrotic human liver tissues to investigate alterations in protein levels and affected pathways among different fibrotic stages. Observed changes in the glycolytic pathway caused by HBx presence and therefore its interactions with HIF-1α can be a target pathway for novel therapeutic purposes. |
| Anahtar Kelimeler |
| Chronic hepatitis B | Glycolysis | Liver fibrosis | Proteomics | Two-dimensional difference gel electrophoresis |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 21 |
| SCOPUS | 23 |
| Google Scholar | 29 |