Simple sequence repeat based assessment of genetic relationships among Prunus rootstocks      
Yazarlar (9)
Z. Turkoglu
Ondokuz Mayis Üniversitesi, Türkiye
S. Bilgener
S. Ercisli
M. Bakir
A. Koc
M. Akbulut
R. Gercekcioglu
Prof. Dr. Mehmet GÜNEŞ Tokat Gaziosmanpaşa Üniversitesi, Türkiye
A. Esitken
Makale Türü Açık Erişim Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Genetics and Molecular Research Gmr
Dergi ISSN 1676-5680 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2010
Cilt No 9
Sayı 4
Sayfalar 2156 / 2165
DOI Numarası 10.4238/vol9-4gmr957
Makale Linki http://www.funpecrp.com.br/gmr/year2010/vol9-4/pdf/gmr957.pdf
Özet
Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of alleles per locus ranged from 8 to 12, with a mean of 9, while the number of microsatellite genotypes varied from 8 to 17, indicating that the SSRs were highly informative. The degree of heterozygosity (0.61) was high. Clustering analysis resulted in two main clusters. The first cluster was divided into two subclusters; the first subcluster consisted of P. avium and P. cerasus, and the second subcluster consisted of P. laurocerasus. The second cluster was divided into two subclusters. The first subcluster consisted of P. mahaleb genotypes and the second consisted of P. angustifolia genotypes. The reference rootstocks also clustered with their associated botanical species. Unweighted pair-group method with arithmetic mean analysis demonstrated that P. laurocerasus genotypes had less genetic variation and that P. avium genotypes were more closely related to P. cerasus. The SSR-based phylogeny was generally consistent with Prunus taxonomy information, suggesting the applicability of SSR analysis for genotyping and phylogenetic studies in the genus Prunus.
Anahtar Kelimeler
Prunus | SSR | Genotyping | Diversity | Rootstock