Exploring of biological activity and diverse metabolites in hemp (Cannabis sativa) seed oil by GC/MS, GC–FID, and LC–HRMS chromatographies
Yazarlar (8)
İlhami Gülçin Atatürk Üniversitesi, Türkiye
Eda Mehtap Ozden
Muzaffer Mutlu Türkiye
Ziba Mirzaee
Dr. Öğr. Üyesi Zeynebe BİNGÖL Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ekrem Köksal Türkiye
Saleh Alwasel
Ahmet Ceyhan Gören Türkiye
| Makale Türü |
Özgün Makale
(ESCI dergilerinde yayınlanan tam makale)
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| Dergi Adı | FUTURE JOURNAL OF PHARMACEUTICAL SCIENCES | ||
| Dergi ISSN | 2314-7245 Wos Dergi | ||
| Dergi Tarandığı Indeksler | ESCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 09-2024 |
| Cilt / Sayı / Sayfa | 10 / 1 / – | DOI | 10.1186/s43094-024-00705-2 |
| Makale Linki | https://doi.org/10.1186/s43094-024-00705-2 | ||
| Özet |
| Background This study investigated the antidiabetic and antioxidant properties of hemp seed oil using various bioanalytical methods. Furthermore, this study determined the suppressive properties of hemp seed oil on α-amylase, acetylcholinesterase and carbonic anhydrase II that purifed by the sepharose-4B-L-Tyrosine-sulfanilamide afnity chromatoghraphy, all of which are related to diferent metabolic diseases. Moreover, the phenolic concentration in the essential oil was quantifed through LC–HRMS chromatography. Thirteen distinct phenolic compounds were detected in hemp seed oil. Additionally, both the chemical components and quantity of essential oils within hemp seed oil were assessed through GC–FID and GC/MS analyses. Results The predominant essential oils in hemp seed oil included linoleoyl chloride (34.62%), linoleic acid (33.21%), and 2-4-di-tert-butylphenol (5.79%). Hemp seed oil’s ability to scavenge radicals was studied through the use of 2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and 1, 1-diphenyl-2-picrylhydrazil bioanalytical radical scavenging methods. The results unveiled its potent radical-scavenging properties, with an 46.20 μg/mL for 2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radicals and IC50 of 9.76 μg/mL for 1, 1-diphenyl-2-picrylhydrazil radicals. The investigation also extended to explore the reducing capabilities of Fe3+-2, 4, 6-tri (2-pyridyl)-S-triazine, copper (Cu2+), and iron (Fe3+). Hemp seed oil demonstrated notable inhibitory efect against α-amylase (IC50: 545.66 μg/mL), achethylcholinesterase (IC50: 28.00 μg/mL), and carbonic anhydrase II (IC50: 322.62 μg/mL). Conclusions … |
| Anahtar Kelimeler |
| Cannabis sativa | Hemp seed oil | Chromatography | Antioxidant activity | Enzyme inhibition | LC-HRMS |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 13 |
| Google Scholar | 14 |