Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties
Yazarlar (10)
F. Kamah Université 20 Août 1955-Skikda, Cezayir
A. Basli Université 20 Août 1955-Skikda, Cezayir
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
A. Bouzana Université 20 Août 1955-Skikda, Cezayir
C. Bensouici Center For Research İn Biotechnology, Cezayir
T. Richard Université De Bordeaux, Fransa
F. A. Al-Mekhlafi College of Sciences, Suudi Arabistan
M. A. Wadaan College of Sciences, Suudi Arabistan
M. S. Al-Khalifa College of Sciences, Suudi Arabistan
F. Boulkenafet Université 20 Août 1955-Skikda, Cezayir
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Arquivo Brasileiro De Medicina Veterinaria E Zootecnia (Q4)
Dergi ISSN 0102-0935 Dergi Bilgileri (2025)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili Türkçe Basım Tarihi 01-2025
Kabul Tarihi – Yayınlanma Tarihi 01-01-2025
Cilt / Sayı / Sayfa 77 / 1 / – DOI 10.1590/1678-4162-13361
Makale Linki https://doi.org/10.1590/1678-4162-13361
UAK Araştırma Alanları
Organik Kimya
Özet
The surge in interest in bioactive plant compounds, particularly from grapes (Vitis vinifera L.), has led to this study on phenolic compounds at different ripening stages. Grapes were collected before veraison, at veraison, and during ripening. Phenolic compounds were extracted using 50% acetone and quantified through spectrophotometric assays for total phenolic, flavonoid, flavanol, condensed tannin, and hydrolysable tannin contents. LCMS/MS identified phenolics. Antioxidant activities were assessed via (DPPH), (ABTS), phenanthroline, reducing power, and silver nanoparticle assays. Additionally, anti-tyrosinase activity and photoprotective effects were evaluated. The results indicated that before veraison (BV) stages yielded the highest concentrations of phenolic compounds. The most abundant compounds at the berry veraison stage were catechin (72645.40, Sabel), gallic acid (1130.75, Red Glob), and luteolin (164.44, Cardinal). Antioxidant activities were consistent with phenolic content trends, with significant tyrosinase enzyme suppression and high SPF factor observed. Principal Component Analysis highlighted strong correlations between phenolic profiles and antioxidant activities. This study is the first to examine how grape ripeness affects phenolic content and biological activities in Algerian grape seeds. It identifies optimal harvest times for maximizing bioactive compounds and supports the use of grape seed extract in sunscreens and antioxidants.
Anahtar Kelimeler
anti-tyrosinase | antioxidant | LCMS/MS | repining stages | SPF factor