Phytochemical Study of Astragalus onobrychis: Bioactive Compounds, Antioxidant Activity, in silico Molecular Docking, PASS and DFT Study
Yazarlar (5)
Yunus Başar Iğdır Üniversitesi, Türkiye
Doç. Dr. İlyas YILDIZ Tokat Gaziosmanpaşa Üniversitesi, Türkiye
İbrahim Hosaflıoğlu Iğdır Üniversitesi, Türkiye
Prof. Dr. Ramazan ERENLER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Mehmet Hakkı Alma Kahramanmaraş Sütçü İmam Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Brazilian Archives of Biology and Technology (Q3) | ||
| Dergi ISSN | 1516-8913 Dergi Bilgileri (2025) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2025 |
| Cilt / Sayı / Sayfa | 68 / 1 / 1–15 | DOI | 10.1590/1678-4324-2025240574 |
| Makale Linki | https://doi.org/10.1590/1678-4324-2025240574 | ||
| UAK Araştırma Alanları |
Organik Kimya
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| Özet |
| Astragalus species include rich bioflavonoids, triterpene glycosides, flavonoids, isoflavonoids and saponins. Astragalus species are traditionally used for diarrhea, dental treatment, diabetes treatment and as a cough remedy. In this study, the phytochemical content (total phenolic content and quantitative analysis of phenolics) and biological activity (DPPH˙) of the methanol extract of Astragalus onobrychis were investigated. The SPMB technique, a new method, was used for total phenolic and DPPH˙ analysis. Density functional theory (DFT) and theoretical in silico studies (molecular docking and PASS) were applied to the major component as kaempferol-3-glucoside (K3G) in the constituent analysis, which was performed by LC-ESI-MS/MS. Accordingly, the methanol extract of A. onobrychis was found to have high total phenolic content (FCR; 111.67 mg GA/g plant) and DPPH˙ (48.57%) analysis. Moreover, LC-ESI-MS/MS analysis revealed the K (285.011 µg/g extract) to be major constituent. In addition, the interactions of the K3G molecule with DNA mtyltransefraze 1 and urease enzyme were compared with the standard daunorubicin and thiourea. It was found that K3G may have inhibitory potential. These results can be substantiated in future studies by in vivo and in vitro studies. |
| Anahtar Kelimeler |
| antioxidant activity | Astragalus onobrychis | kaempferol-3-glucoside | LC-ESI-MS/MS |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 10 |
| Scopus | 10 |
| Google Scholar | 17 |