Streptomyces sp TEM 33 possess high lipolytic activity in Solid State Fermentation in comparison with Submerged Fermentation
Yazarlar (3)
Prof. Dr. Bilge Hilal ÇADIRCI
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY |
| Dergi ISSN | 1082-6068 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2016 |
| Cilt No | 46 |
| Sayı | 1 |
| Sayfalar | 23 / 29 |
| DOI Numarası | 10.1080/10826068.2014.970693 |
| Özet |
| Solid-state fermentation (SSF) is a bioprocess that doesnt need an excess of free water, and it offers potential benefits for microbial cultivation for bioprocesses and product development. In comparing the antibiotic production, few detailed reports could be found with lipolytic enzyme production by Streptomycetes in SSF. Taking this knowledge into consideration, we prefer to purify Actinomycetes species as a new source for lipase production. The lipase-producing strain Streptomyces sp. TEM 33 was isolated from soil and lipase production was managed by solid-state fermentation (SSF) in comparison with submerged fermentation (SmF). Bioprocess-affecting factors like initial moisture content, incubation time, and various carbon and nitrogen additives and the other enzymes secreted into the media were optimized. Lipase activity was measured as 1.74 ± 0.0005 U/g dry substrate (gds) by the p-nitrophenylpalmitate (pNPP) method on day 6 of fermentation with 71.43% final substrate moisture content. In order to understand the metabolic priority in SSF, cellulase and xylanase activity of Streptomyces sp. TEM33 was also measured. The microorganism degrades the wheat bran to its usable form by excreting cellulases and xylanases; then it secretes the lipase that is necessary for degrading the oil in the medium. |
| Anahtar Kelimeler |
| Cellulase activity | lipase | solid-state fermentation | Streptomyces | Xylanase activity |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 9 |
| SCOPUS | 10 |
| Google Scholar | 16 |