An Organic Solvents Tolerant and Thermotolerant Lipase from Pseudomonas fluorescens P21
Yazarlar (2)
Prof. Dr. Bilge Hilal ÇADIRCI Tokat Gaziosmanpaşa Üniversitesi, Türkiye
İhsan Yaşa Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (Q4) | ||
| Dergi ISSN | 1381-1177 | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2010 |
| Cilt / Sayı / Sayfa | 64 / 3 / 155–161 | DOI | 10.1016/j.molcatb.2009.09.009 |
| Makale Linki | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGN-4X9FGYB-1&_user=1061107&_coverDate=07%2F31%2F2010&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1646975255&_rerunOrigin=google&_acct=C000051152&_ve | ||
| Özet |
| In this study, 38 organic solvents tolerant bacteria have been isolated from 21 different soil and water environments and the bacterium called P21 has been isolated among them due to its ability of surviving in 30% toluene, which is highly toxic to microorganisms and its capability of high lipase production (3.5U/l). The lipase production medium has been optimized and the medium containing olive oil as an inducer and peptone as a nitrogen source has been selected as the best ingredients for the highest lipase production conditions (15U/l). After this optimization, initial lipase activity has increased to 638.3%. In our study, to identify the physical and molecular properties of the lipase, the supernatant has been obtained at the highest production value in early stationer phase of the P21's growth curve and the supernatant has concentrated by ultrafiltration and ammonium sulphate precipitation. SDS-PAGE and … |
| Anahtar Kelimeler |
| Extracellular lipase | Organic solvents tolerance | Pseudomonas fluorescens |