Thermostable Serine Protease By Aspergillus parasiticus TEM Have Promising Activity In Enzymatic Dehairing Process
Yazarlar (4)
Prof. Dr. Bilge Hilal ÇADIRCI Tokat Gaziosmanpaşa Üniversitesi, Türkiye
İhsan Yaşa Ege Üniversitesi, Türkiye
Behzat Oral Bitlisli Ege Üniversitesi, Türkiye
Hüseyin Ata Karavana Ege Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
|
||
| Dergi Adı | Journal of New Results in Science | ||
| Dergi ISSN | 1304-7981 | ||
| Dergi Tarandığı Indeksler | getCITED, Directory of Research Journal Indexing (DRJI), ResearchBib, Google Scholar | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2015 |
| Cilt / Sayı / Sayfa | – / 8 / 72–83 | DOI | – |
| Makale Linki | http://dergipanel.gop.edu.tr/dosya/dergi_5/yayimlananlar/yil_2015_sayi_8/JNRS150808.pdf | ||
| UAK Araştırma Alanları |
Mikrobiyoloji
|
||
| Özet |
| In the present study, Aspergillus parasiticus TEM obtained from our culture collection produced appreciable quantities (2955.739.4 U/gds) of extracellular protease, but negligible quantities of keratinase, when grown on wheat bran moistened to 75% with Czapex Dox solution containing 1% NaNO3 by means of solid-state fermentation (SSF). After incubation at 27C for 3 days, A. parasiticus TEM reached to a maximum protease activity. The optimum pH of purified enzyme was around pH 8.0 at 30C, but substantial activity was recorded at the pH 7.0-11.0 and it is stable pH values ranging from 7.0-9.0 for 8 hours. Temperature optimum of the enzyme was found to be at 50 oC without Ca++ and to be highly stable (90%) at 30oC for 8 hours. When the protease from A. parasiticus TEM was used in dehairing process of cattle hide, the mixture of 100% crude enzyme, 1.5% lime and 1% sodium sulphide based on hide weight was completely dehaired hides at 30oC for 6 hours, but crude enzyme 200% alone carried out this process for 8 hours |
| Anahtar Kelimeler |
