Development and Validation of a LC Method for the Analysis of Phenolic Acids in Turkish Salvia Species

Kan, Y.; Gökbulut, A.; Kartal, M.; Konuklugil, B.; YILMAZ, GÜLDEREN

doi:10.1365/s10337-007-0278-7

Development and Validation of a LC Method for the Analysis of Phenolic Acids in Turkish Salvia Species

Yazarlar (5)

Y. Kan
Selçuk Üniversitesi, Türkiye

A. Gökbulut
Ankara Üniversitesi, Türkiye

M. Kartal
Ankara Üniversitesi, Türkiye

B. Konuklugil
Ankara Üniversitesi, Türkiye

Doç. Dr. Gülderen YILMAZ Ankara Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Chromatographia
Dergi ISSN	0009-5893 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	07-2007
Cilt / Sayı / Sayfa	66 / 0 / –	DOI	10.1365/s10337-007-0278-7
Makale Linki	http://link.springer.com/10.1365/s10337-007-0278-7
UAK Araştırma Alanları	Farmasotik Botanik

Özet

An accurate, simple, reproducible, and sensitive method for determination of rosmarinic, caffeic, chlorogenic, and gallic acids in 12 Salvia species growing naturally in Anatolia, has been developed and validated. The phenolic acids were separated using a μBondapack C18 column by gradient elution with a flow rate of 1.0 mL min^-1, which was adjusted to deliver firstly o-phosphoric acid 0.085% in water, 0.085% in methanol, and 0.085% in 2-propanol (80:10:10, v/v/v), then decreased gradually (60:20:20, v/v/v) during 20 min with a flow rate of 1.0 mL min^-1. The samples were monitored at 220 nm for gallic acid and 330 nm for rosmarinic, caffeic, and chlorogenic acids using photo-diode array detection. The linear range of detection for gallic, chlorogenic, caffeic, and rosmarinic acids were between 0.051-101.4, 0.207-103.6, 0.100-100, and 0.201-100.5 μg mL^-1, respectively. The linearity, range, peak purity, selectivity, system performance parameters, precision, accuracy, and robustness had also acceptable values. The developed method was applied to the flower, leaf, stem, and root parts of the Salvia species. © 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.

Anahtar Kelimeler

Column liquid chromatography | Phenolic acids | Rosmarinic and caffeic acids | Salvia

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Scopus	27

Development and Validation of a LC Method for the Analysis of Phenolic Acids in Turkish Salvia Species

Dergi Adı	CHROMATOGRAPHIA
Yayıncı	Springer
Açık Erişim	Hayır
ISSN	0009-5893
E-ISSN	1612-1112
CiteScore	2,7
SJR	0,278
SNIP	0,492

Development and Validation of a LC Method for the Analysis of Phenolic Acids in Turkish Salvia Species

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