Purification and determination of inhibitory activity of recombinant soyacystatin for surimi application
Yazarlar (2)
Prof. Dr. Özlem AKPINAR Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Haejung An
University Of Southern California, Amerika Birleşik Devletleri
University Of Southern California, Amerika Birleşik Devletleri
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Molecular Nutrition and Food Research | ||
| Dergi ISSN | 1613-4125 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2005 |
| Cilt / Sayı / Sayfa | 49 / 3 / 247–255 | DOI | 10.1002/mnfr.200400059 |
| Makale Linki | http://doi.wiley.com/10.1002/mnfr.200400059 | ||
| UAK Araştırma Alanları |
Gıda Kimyası
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| Özet |
| A recombinant soyacystatin (r‐soyacystatin) was tested for its inhibitory activity against cysteine proteinase of Pacific whiting and its activity was compared to that of egg white cystatin. A recombinant soyacystatin expressed in Escherichia coli was purified to electrophoretic homogeneity using phenyl‐Sepharose and DEAE‐Sepharose. Native egg white cystatin was purified by using affinity chromatography on CM‐papain‐Sepharose generated in our lab. Egg white cystatin and soyacystatin were tested for proteinase inhibitory activity against commercial papain and also cathepsin L purified from Pacific whiting muscle. The r‐soyacystatin exhibited papain‐like protease inhibition activity comparable to that of the egg white cystatin, which could inhibit papain and Pacific whiting cathepsin L. The r‐soyacystatin subsequently inhibited the autolytic activity of Pacific whiting surimi. |
| Anahtar Kelimeler |
| Autolytic activity | Egg white cystatin | Pacific whiting | Soyacystatin | Surimi |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 4 |
| Scopus | 4 |
| Google Scholar | 8 |