Determination of Bio-Variation Among Different Mulberry Species Grown in Tokat Region of Turkey By Molecular Markers
Yazarlar (2)
Prof. Dr. Çetin ÇEKİÇ
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Middle Black Sea Transitional Zone Agricultural Research Institute, Türkiye
| Makale Türü |
Özgün Makale
|
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Applied Ecology and Environmental Research |
| Dergi ISSN | 1589-1623 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2018 |
| Cilt No | 16 |
| Sayı | 4 |
| Sayfalar | 4523 / 4530 |
| DOI Numarası | 10.15666/aeer/1604_45234530 |
| Özet |
| The aim of this research is to determinate diversity of mulberries grown in North Anatolia between or within species by ISSR markers. In the study, 38 different mulberry genotypes collected from Tokat region in Turkey were used. ISSR-PCR analyses were carried out on the DNA of genotypes isolated using mini-CTAB extraction method. The levels of polymorphism between genotypes were determined using the UBC-ISSR primers. A total of 96 bands were obtained from 15 UBC ISSR primers. Out of 96 bands, 80 bands were polymorphic. The number of bands obtained per primer ranged between 4 and 11, the average number of bands were determined as 6.4. The average number of polymorphic bands per primer was 5.33. Similarities and differences between genotypes have been studied at the molecular level. The data used for statistical analysis were obtained by the evaluation of ISSR bands. Similarity coefficient and UPGMA dendrogram were built using the Basic Coordinates Analysis. According to the dendrogram, the genotypes have been divided in two main groups, one small and one large group. While the small group was only comprised of black mulberry genotypes, the large group included white mulberry, weeping mulberry and wild white mulberry genotypes. Different mulberry species have been divided into different subgroups within the large group. The polimorfism level within the species was lowest in black mulberry genotypes, and this was followed by weeping mulberry, white mulberry and wild white mulberry genotypes, respectively. |
| Anahtar Kelimeler |
| Dendrogram | Diversity | Mulberry | North anatolia | Similarity |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| SCOPUS | 1 |
| Google Scholar | 1 |