Genetic Diversity of Wild Raspberry Genotypes in North Anatolia Based on ISSR Markers     
Yazarlar (3)
Prof. Dr. Çetin ÇEKİÇ Tokat Gaziosmanpaşa Üniversitesi, Türkiye
O. Calis
Akdeniz Üniversitesi, Türkiye
S. Ozturk Erdem
Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Applied Ecology and Environmental Research
Dergi ISSN 1589-1623 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 10-2018
Cilt No 16
Sayı 5
Sayfalar 6835 / 6843
DOI Numarası 10.15666/aeer/1605_68356843
Makale Linki https://www.aloki.hu/indvol16_5.htm
Özet
The preliminary aim of the study before future breeding program was to reveal usability of ISSR markers to assess genetic diversity and relatedness within raspberry genotypes wildly grown in Northern Turkey. In this study, 19 wild raspberry genotypes collected from Black Sea region in Turkey were used. ISSR PCR analyses were carried on the DNA of genotypes isolated using mini CTAB extraction method. The levels of polymorphism between genotypes were determined using the UBC ISSR primers. A total of 111 bands were obtained from 15 UBC ISSR primers. Out of 111 bands, 101 bands were polymorphic. The number of bands obtained per primer ranged between 3 and 10, the average number of bands were determined as 7.4. The average number of polymorphic bands per primer was 6.73. Similarities and differences between genotypes have been studied at the molecular level. The data used for statistical analysis were obtained by the evaluation of ISSR bands. Similarity coefficient and UPGMA dendrogram were built using the Basic Coordinates Analysis. According to the dendrogram, the genotypes have been divided in two main groups, one small and other large group. While the small group was only comprised of three (A8, A10 and A14) genotypes, the large group included the rest of the genotypes studied.
Anahtar Kelimeler
Dendrogram | Molecular | Polymorphism | Raspberry | Turkey