Evaluation of Process Conditions for Ultrasonic Spray-Freeze Drying of Transglutaminase
Yazarlar (2)
Prof. Dr. Hilal İŞLEROĞLU Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Arş. Gör. İzzet TÜRKER Tokat Gaziosmanpaşa Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | FOOD TECHNOLOGY AND BIOTECHNOLOGY (Q2) | ||
| Dergi ISSN | 1330-9862 Dergi Bilgileri (2020) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2020 |
| Cilt / Sayı / Sayfa | 58 / 1 / – | DOI | 10.17113/ftb.58.01.20.6544 |
| Makale Linki | https://www.ftb.com.hr/images/InPress/6544-in_press.pdf | ||
| UAK Araştırma Alanları |
Gıda Teknolojileri
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| Özet |
| In this study, a commercial transglutaminase enzyme was dried using an ultrasonic spray freeze drying method and the effects of the process conditions were optimized to maximize the final transglutaminase activity. Accordingly, process parameters affecting enzyme activity were selected, such as nozzle frequency (48 and 120 kHz), flow rate (2, 5 and 8 mL/min) and plate temperature for secondary drying (25, 35 and 45 °C). Moreover, the effects of different pH values (pH=2.0 and pH=9.0) and high temperature (80 °C) on enzyme activity, physical properties and particle morphology of transglutaminase were discussed. According to the results, transglutaminase preserved its activity despite ultrasonic spray freeze drying. Sonication enhanced the enzyme activity. Using the desirability function method, the optimum process conditions were determined to be flow rate 3.10 mL/min, plate temperature 45 °C and nozzle frequency 120 kHz. The predicted activity ratio was 1.17, and experimentally obtained ratio was 1.14±0.02. Furthermore, enzyme produced by ultrasonic spray freeze drying had low moisture values (2.92-4.36%) at 8 h of drying. When the morphological structure of the transglutaminase particles produced by ultrasonic spray freeze drying under the optimum conditions was examined, spherical particles with pores on their surfaces were observed. In addition, flow properties of the transglutaminase powders were considered as fair under most conditions according to the Carr index. |
| Anahtar Kelimeler |
| enzyme activity | flow behaviour | particle morphology | spray freeze drying | transglutaminase | ultrasonication |
BM Sürdürülebilir Kalkınma Amaçları
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| Yöksis | 1 |
| PubMed | 1 |